The spectral range is from 430 to 930 nm with spectral resolution of approximately 10 nm (full width at half maximum) and spatial resolution better than 1 mm. Our system is capable of reflectance and fluorescence measurements with the use of dual illumination sources where fluorescence emissions are measured with ultraviolet (UV-A) excitation. We present the calibrations and image-correction procedures for the system artifacts and heterogeneous responses caused by the optics, sensor, and lighting conditions throughout the spectrum region for reflectance and fluorescence. The results of the fluorescence correction method showed that the system responses throughout the spectrum region were normalized to within 0.5% error. The versatility of the hyperspectral imaging system was demonstrated with sample fluorescence and reflectance images of a normal apple and an apple with fungal contamination and bruised spots. The primary use of the imaging system in our laboratory is to conduct food safety and quality research. However, we envision that this unique system can be used in a number of scientific applications.