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Strain Сhlorella sp. IPPAS C-1210 is an effective lipid and triacilglycerols (TAG) producer. The strain could be used eventually in such industries as bioenergetics, food industry and agriculture. The objective of this work was investigation of conditions in which the strain Сhlorella sp. IPPAS C-1210 accumulates the most starch and TAG in cells with a view to optimise its growth and productivity. The following cultivation parameters were investigated in order to figure out their influence on accumulation of starch and TAG: nitrogen- and phosphorous-starvation and cultivation on media with different nitrogen (nitrate, urea) and carbon (carbon dioxide, bicarbonate) sources. Pigments, starch, protein and lipid content in cells were measured. The exclusion of nitrogen or phosphorus source from medium decreased the biomass productivity significantly, caused chlorosis and reduction of protein content. Total lipid content increased slightly after phosphorous starvation and stayed almost constant under nitrogen starvation, however a greater TAG increase was observed during nitrogen starvation. Both nitrogen and phosphorous starvations caused the increase of the amount of reserve carbohydrates: during phosphorous starvation increase was insignificant, whereas the latter almost doubled the amount of reserve carbohydrates. The highest biomass and lipid productivity was observed in cells grown in bicarbonate supplement medium and the highest starch productivity was observed in cells grown in standard BBM-3N medium.

As a result of screening of spore-forming bacteria, a B-13186 strain with a wide spectrum of antagonistic activity identified as Brevibacillus laterosporus by the analysis of sequences of variable sites of 16S rRNA was selected. Morphological, cultural and biochemical characteristics of the strain were studied. A distinctive feature of the strain is the presence of a canoe-like inclusion formed in sporangia and attached to the mature spore, as well as the ability to synthesize round-shaped crystalline inclusions. The strain was shown to be active against various species of gram-positive bacteria including A search for strains capable of simultaneously producing high amounts of several biologically valuable compounds and/or having high biomass productivity has been carried out. The growth characteristics and biochemical composition of 12 microalgae and cyanobacteria strains from the IPPAS Collection in the exponential and stationary growth phases were studied. All the strains had high growth rates (doubling time 6-22 h). The strains of Cyanobacterium sp. IPPAS B-1200, Chlorella sp. IPPAS C-1210, Nannochloris sp. IPPAS C-1509, Cyanidium caldarium IPPAS P-510 and Vischeria sp. IPPAS H-242 demonstrated the highest biotechnological potential and can be used for the production of various types of biofuel, pigments, feed and food additives, including those with high content of eicosapentaenoic (20:5 Δ5,8,11,14,17) acid content. microalgae, cyanobacteria, biochemical composition, fatty acids, valuable metabolites, growth characteristics This work was supported by a grant from the Russian Science Foundation [no. 14-14-00904]

Пленарные доклады 1Казанский институт биохимии и биофизики -обособленное структурное подразделение ФИЦ КазНЦ РАН, Казань, Россия 2Казанский (Приволжский) федеральный университет, Казань, Россия, 3Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии растений и микроорганизмов РАН, Саратов, Россия, 4Институт органической и физической химии им. А.Е. Арбузова -обособленное структурное подразделение ФИЦ КазНЦ РАН, Казань, Россия