Measurement of platelet function by light transmission aggregometry (LTA) using a special device – an aggregometer requires significant time and is time-consuming. In this study, an automated LTA procedure was evaluated to establish the reference ranges. On the Sysmex CS-5100 analyzer, aggregation measurements were performed using several agonists at a certain concentration: ADP (2 µmol/L); arachidonic acid (1 mmol/L); collagen (2 µg/ml); ristocetin (1.2 mg/ml); epinephrine (5 µmol/L). For each agonist, the maximum and final aggregation, the Lag phase and the Area under the aggregation curve were measured. Reference ranges for a standard panel of activators were determined on 40 samples of healthy subjects in the concentrations recommended by the International Society on Thrombosis and Haemostasis. A standard panel of agonists can be used on Sysmex CS series analyzers: ADP, arachidonic acid; collagen; ristocetin, epinephrine, so these devices can replace specialized aggregometers or perform platelet aggregation where this investigation is not currently performed.

The article defines reference values for activated partial thromboplastin time, Quick’s value, INR, thrombin time, fibrinogen, antithrombin and II, V, VII, VIII, IX, X, XI and XII coagulation factors, according to existing standards on the automated Sysmex CS‑2000i analyzer.The aim of the study. To determine reference values for routine and specific parameters of the hemostasis, which may vary depending on the type of analyzer and utilized reagents.Materials and methods. After receiving informed consent from donors for medical survey and blood donation, blood samples were obtained from 100 healthy donors: 64 (64%) males и 36 (36%) females. We established reference values with the Sysmex CS‑2000i (Sysmex, Japan) hemostasis analyzer and reagents from Siemens (Siemens Healthcare, Germany).Results. The data obtained were compared with the literature data and the data presented in the instructions for the reagents used. The results obtained for activated partial thromboplastin time (23.59–35.69 sec), fibrinogen (1.67–3.59 g/l) and antithrombin (67.65–114.89%) are comparable to the available data. There are no data on other studied parameters of hemostasis for the Sysmex CS‑2000i analyzer and the reagents used in the work. The obtained reference intervals are consistent with the recommendations of the manufacturer.Conclusions. Reference values vary significantly depending on the analytical systems and reagent kits used, which confirms the need for local derivation or validation of reference intervals for each specific analytical system and in each laboratory.

Laboratory tests for lupus anticoagulant (LA) are necessary for antiphospholipid syndrome (APS) diagnosis. Due to antibody heterogeneity, there are two test systems recommended to maximize detection rates: one of them is the dilute Russell’s viper venom time (dRVVT) and the second one is the activated partial thromboplastin time (APTT). Current guidelines recommend getting the reference ranges and cut-off of the normalized screen/confirm ratio in dRVVT and APTT in every laboratory. The aim of this work is establishing of reference ranges and cut-off for the normalized ratio in dRVVT and APTT reagents using two type of sample preparation. The cut-off values of LA1/LA2 (37 °C, 30 minutes), LA1/LA2 (22–25 °C, 30 minutes), Actin FSL/Actin FS (37 °C, 30 minutes) and Actin FSL/Actin FS (22–25 °C, 30 minutes) pairs in the normalized ratio were 1.21, 1.18, 1.07 and 1.08, respectively.