Rotavirus have been reported as the principal causal pathogen of acute gastroenteritis and one of the leading causes of child morbidity and mortality in Egypt .This study aimed to investigate biological , serological and molecular Rotavirus genotypes infection in Egypt . The blood and stool clinical specimens were collected randomly from 100 patients .They were analyzed for the presence of antigen and the antibody using enzyme-linked immunosorbent assay (ELISA). Rotavirus antigen in stool and antibodies in serum were detected by ELISA among Children (78.13); Female (12.5 ) and male (9.38%). Rotavirus titer was determinated as 5.5x10 7 ; 6.0x10 6 and 7.5x10 7 (PFU\ml) on vero cell of Female ; male and children respectively after 4 days Post infection. The cytopathic effects were granulation of cytoplasm , enlarged , swelling , rounded and multinucleated giant cells .The virus particles are non-enveloped with Icosahedral symmetry with about 70 nm in diameter .Rotavirus isolates showed homologous with RV isolates recorded in gene bank based on alignment and phylogenetic tree analysis. Six RV genotypes (G1P4, G1P6, G1P8 , G9P6 , G1P8, G3 P8) were detected in summer. Seven RV genotypes, (G1P4, G1P6, G1P8 , G3P4 , G3P6, G3P8 G9P8) were detected in in spring and five RV genotypes (G1P4, G1P8 , G3P4, G3P8 G9P8) were detected in winter seasons . SDS-PAGE showed seven structural and function of proteins were polymorphism variation among for Rotavirus isolates . RV genomic migration pattern of G1P4, G1P6, G1P8 , and G9P8 were varied in number , density and segmented to 12 , 9 , 10 , and 9 moved respectively .

Background: obstetric emergencies are life threatening medical condition that occurs during pregnancy, labor, or in the postpartum period. Moreover, internship offer carefully planned, monitored work experience which allow the opportunity to apply knowledge and skills in a professional setting. Aim: to evaluate the effect of emergency obstetric protocol on the performance of intern nursing students. Subjects and Methods: a quasi experimental design was utilized. Sample: a convenient sample, all female intern nursing students (100) in the technical institute of nursing, faculty of nursing Ain-Shams University in the academic year (2019/2020) and Purposive sample, 59 conscious women with obstetric emergencies who agreed to participate in the study and with non co-morbid condition. Setting: The study was conducted in technical institute of nursing faculty of nursing and in emergency unit, intensive care unit and labor unit of maternity hospital Ain-Shams University. Tools: Tool 1 self-administered structured questionnaire sheet, Tool 2 observational checklist Tool 3 maternal satisfaction questionnaire sheet. Results: showed statistically significant improvements in intern nurses' knowledge and practical skills related to emergency obstetric care immediately post protocol intervention and in follow up phase and increase women satisfaction level toward care provided by intern nurse, study confirmed statistically significant positive correlation between intern nurse's knowledge and practice related to emergency obstetric care post intervention and statistically significant positive correlation between intern nurse's knowledge, practice and women satisfaction post protocol intervention. Conclusion: Implementing emergency obstetric protocol had a positive effect on the performance of intern nursing students. Recommendations: Dissemination of emergency obstetric protocol for all intern nursing students in technical nursing institutions this will be effective in improving their performance.