Cholinesterase (EC 3.1.1.8) for intravenous use has been obtained from fraction
IV, a by-product of ethanol fractionation. A four-step procedure has been developed
associating precipitation by Rivanol® and PEG 4000 with chromatography on DEAE-cellulose.
The yield corresponds to 30-35% of the activity found in fraction IV. Vials containing
5000 U of CHE stabilized by 2.5% albumin have been prepared. The pyrogen-free
product is well tolerated in the human.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.