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Nucleic acidsNucleic acids U 0700 Synthesis and Properties of 2'-O,4'-C-Ethylene-Bridged Nucleic Acids (ENA) asEffective Antisense Oligonucleotides. -(MORITA, K.; TAKAGI, M.; HASEGAWA, C.; KANEKO, M.; TSUTSUMI, S.; SONE, J.; ISHIKAWA, T.; IMANISHI, T.; KOIZUMI*, M.; Bioorg. Med. Chem. 11 (2003) 10, 2211-2226; Explor. Chem. Res. Lab.,

Dynamic CHP-CD nanogels, which consisted of a self-assembly of cholesteryl-group-bearing pullulan (CHP) and beta-cyclodextrin (CD), were characterized by SEC and SEC-MALS methods. The nanogels prevented the thermal aggregation of carbonic anhydrase B (CAB) by selective trapping of the heat-denatured protein. After the complex between the CHP-CD nanogels and CAB was cooled, the enzyme activity of CAB spontaneously recovered upon release from the complex. The dynamic nanogels self-regulated an association of heat denatured protein and dissociation of native protein depending on the concentration of CD. The thermal stability of CAB was improved by thermoresponsive controlled association between the proteins and the artificial molecular chaperone.