Preoperative preparation of infected wounds of superficial tissues, using the method of hyperbaric oxygenation with the ozone-containing mixture

Мета. Розробка нової технології профілактики ускладнень та покращення загоювання післяопераційних ран під впливом стимуляції оптичним потоком червоного (635 нм) і синього (450 нм) діапазонів спектра. Матеріали і методи. Дослідження проведені на 18 білих щурах, яким під загальним внутрішньочеревним наркозом виконували розтин шкіри черевної стінки та підшкірної клітковини, рану зшивали вузловими швами. Після цього у 9 тварин (контрольна група) більше не виконували ніяких маніпуляцій, а 9 іншим тваринам (експериментальна група), проводили фототерапію оптичним потоком червоного і синього діапазонів спектра. На 7, 14-ту та 21-шу добу проводили гістологічні дослідження ран черевної стінки. Результати. Новоутворена рубцева тканина дозрівала швидше у тварин експериментальної групи з формуванням вузького нормотрофічного рубця. У тварин контрольної групи більш повільно дозрівав та формувався нерівномірний за товщиною й шириною гіпертрофічний рубець. Висновки. Під впливом опромінення зони ран черевної стінки вони більш швидко та якісно загоювалися.

Objective. To study in experiment the impact of the impulse electrophoresis of the blood plasma method on the wounds healing process in postoperative wounds. Materials and methods. The investigations were conducted on 15 white rats, to which in aseptic environment under intraabdominal narcosis the hip surgical access through the skin and muscles was conducted with subsequent layered closure of the wound, using separate knots. Ten laboratory animals were included into the main group, in whom transdermal introduction of the blood plasma, using the impulse electrophoresis method, was conducted on the 4th, 7th, 10th and 14th postoperative days. Into a control group 5 white rats were included, in which transdermal introduction of the blood plasma was not applied postoperatively. In all the laboratory animals the wounds have healed primarily. On the19th day postoperatively the laboratory animals were extracted from the experiment, and the near suture skin was investigated, using histological methods. Results. The surgical access healing in the hip skin and muscles with formation of normotrophic cicatrices was observed in the main group of the laboratory animals. Hypertrophic cicatrix in the operative intervention zone have been formatted in the laboratory animals of a control group on the 19th postoperative day. Conclusion. More qualitative healing of the wounds with formation of normotrophic cicatrix while application of transdermal introduction of the blood plasma, using the impulse electrophoresis method, was confirmed in experimental investigation in laboratory animals of the main group. Excessive creation of collagen was omitted in the laboratory animals of the main group, what have promoted formation of more thin and tender cicatrix, which have become slightly visible up to the end of experiment.

Objective. Comparison, in conditions of experiment between impact of the blood plasm, enriched by thrombocytes, on the terms of the burn and scalped wounds healing and morphological characteristics of the cicatrices formatted in its injectional or noninjectional introduction, using the method of electrophoresis. Materials and methods. The investigations were conducted on 30 white rats, which were divided into three groups with 10 animals in every group, median body mass of the animals have constituted (275.5 ± 15.1) gr. After achievement of medicinal sleep (intraperitoneal introduction of 1.0 ml of 0.5% solution of sodium thiopental in combination with 0.2 ml of propofol 1% solution the scalped skin incisions with the 1.5 × 0.5 cm dimensions were performed to the animals. Besides that, one of the incisions was processed with diathermy up to the scab crust formation. Both wounds were left open, without sutures. To the control group animals after the wounding conduction no procedures were performed, the wounds have been healed in primary pattern. To the Group I animals during two consequent weeks the blood plasm, enriched by thrombocytes, was introduced, using intracutaneous injections twice a week in quantity of 10 ml, puncturing the injured zone around. To the investigation Group II animals the electrophoresis in accordance to own procedure through napkin, soaked in the blood plasm, enriched by thrombocytes, was performed with the same rate. The blood plasm, enriched by thrombocytes, was prepared from the whole venous blood of human Group A(0), using centrifugation for achievement of targeted quantity of thrombocytes 850 000 in 1 ml. Morphological features of cicatrices formatted and their width were studied, using the methods of light microscopy and morphometry on the 21th day of experiment. Results. Application of the blood plasm, enriched by thrombocytes, was accompanied by statistically significant reduction of the cicatrices width, which were formatted on the 21-th day in locations of the scalped wounds simulation (p < 0.05 and p < 0.01 accordingly, for injectional and electrophoretic methods of application in comparison with the control group) and the burn (p < 0.01 and p < 0.005, accordingly, for the injectional and electrophoretic methods of application in comparison to the control group) wound. At the same time the cicatrices width in electrophoretic application of the blood plasm, enriched by thrombocytes, was statistically significantly lesser (p < 0.05), than in its injectional introduction, not depending on the wound type. Besides that, the cicatrices connective tissue while application of the blood plasm, enriched by thrombocytes, in a moment of the animals evacuation from the experiment have looked like more mature and differed by enhanced organization of the collagen fascicles localization. Conclusion. The method of noninjectional introduction of the blood plasm, enriched by thrombocytes, which was proposed, owes certain advantages over its standard injectional application, which constitute the enhanced degree of maturation of connective tissue, more organized localization of the collagen fascicles, lesser width of the cicatrices evolved and the procedure painlessness. Noninjectional introduction of the blood plasm, enriched by thrombocytes, using method of electrophoresis is free from postinjectional complications, typical for standard methods of its introduction.

Objective. Determination of the virus-cidal and bacterio-cidal action of the ozone-containing steam-water mixture, which was obtained, using apparatus POS-1, simulated on viral models of transmissive gastroenteritis of pigs of the coronaviruses family and polyresistant clinical strains of bacterial cultures, as well as adjustment of optimal parameters of ozone in the gaseous mixture content, which do not cause pathological changes in the organism’s organs and systems. Materials and methods. Apparatus POS-1, created by collective of Scientific-Methodical Centre «Medical Innovation Technologies»», was applied for production of the ozone-containing gaseous mixture. Virusological investigations were conducted on the base of the Institute of Epidemiology and Infectious Diseases named after L. V, Gromashevskiy. As a working material the models of virus of the pigs transmissive gastroenteritis of the coronaviruses family were applied. The series of bacteriological and experimental investigations were conducted on the base of Shalimov National Institute of Surgery and Transplantology NAMS of Ukraine. Polyresistant cultures in concentration of 108 colony-creating units per 1 ml were applied for bacteriological investigations. The experiment was conducted on white rats to study the gaseous ozone-containing mixture affection on living biological tissues. Results. Virusological investigations have shown, that in the 20 minutes exposition and a contact with extracellular virus the infection titer have lowered in 100 000 times, and in a 30 minutes exposition - a complete deactivation of virus have had occurred. Bacteriological investigations have revealed the complete absence of the cultures development after their processing with the ozone-containing mixture during 20 min. The results of swimming tests and behavioral reactions in white rats of control and experimental groups did not differ. Histological investigations of the respiratory organs tissues as well as of spleen, thyroid gland, kidneys and suprarenal glands did not reveal pathological effects of the ozone-containing mixture. Conclusion. Parameters of the ozone generation and delivery in the gaseous mixture content were studied and optimally selected. Investigations of the ozone-containing mixture affection on a viral strain of the coronaviruses family, as well as bacteriological investigations on polyresistant bacterial cultures have had confirmed its pronounced virus-cidal and bactericidal properties. Application of the ozone-containing mixture do not cause any pathological changes in the living organism organs and systems. This gaseous mixture may be used for prevention and treatment of respiratory infectious diseases of viral and bacterial genesis.