No abstract

Diabetes Mellitus is a metabolic disorder characterized by elevated blood glucose level caused due to deficiency of insulin secretion or insulin action. The management of the blood glucose level is an important approach in the control of diabetes mellitus complications. There are many divergent therapeutic strategies in the management of Type II diabetes. The inhibition of carbohydrate hydrolyzing enzymes such as α-amylase and α-glucosidase can be used to lower postprandial blood glucose levels. The objective of the present study is to evaluate the inhibitory potential of Gymnemic acid in various concentrations on α-amylase and α-glucosidase activities. Gymnemic acid and voglibose show the IC50 value of 68.12 and 46.23 on alpha amylase and IC50 value of 65.77 and 36.67 on alpha glucosidase activity respectively. Insilico docking study between α-amylase and α-glucosidase with gymnemic acid and voglibose depict the similar amino acid binding sites that substantiate that gymnemic acid might be used as a natural and cost-effective alternative to the synthetic drug. This study throws light on the anti-diabetic action of phytomedicine, Gymnemic acid, a component of Gymnema sylvestre.

Milky White Mushroom is a species of edible mushroom native to India. The Purpose of this study was to determine the anti-inflammatory and anticancer compounds from Calocybe indica. The ethanolic extract from Calocybe indica were prepared. GC-MS was performed and the major bioactive compounds such as polysaccharides, amino acids, sterols, phytol, and squalene were determined. Calocybe indica was analyzed for their antioxidant activity through In vitro assays such as Free radical activity on DPPH, Hydroxyl radical scavenging assay, Nitric Oxide scavenging assay, Superoxide scavenging assay. Anti-inflammatory effect was estimated by protein denaturation inhibition assay, Proteinase inhibition assay, Cyclooxygenase inhibition assay and Lipoxygenase inhibition assay. Anticancer activity of Calocybe indica extract showed maximum inhibition of 69.11% of growth of human breast cancer cell (MCF 7) at 100μg/ml exposure for about 72 hours. At the end of this study, it was indicated that ethanolic extract of Calocybe indica can be used as an anti- inflammatory and anticancer agent.