Aims: The aim of this study was to determine the effect of different watering regimes on selected coffee genotypes and to evaluate drought tolerance among selected coffee genotypes. The study also aimed at determining the optimum watering interval for these genotypes under high temperature conditions. Study Design: Completely Randomized Design (CRD) with 3 replications. Place and Duration of Study: The experiment was conducted in a greenhouse at Coffee Research Station in Ruiru, Kenya between October and December 2012. Methodology: A total of 11 coffee genotypes were used in this study. These comprised of Coffea canephora (Robusta), one wild accession and nine Coffea arabica genotypes. Six months old seedlings of the test genotypes pre-germinated and transplanted in black polythene bags were obtained from the Coffee Research Station experimental nursery. The seedlings were placed on raised benches in the green house and each potted seedling represented a plot. The seedlings were watered with 0.3 liters of water per pot at six watering regimes applied as follows: watering after every 2 days, 4 days, 7days, 14