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SUMMARY Collagens type I, II, III, IV, and V and the minor cartilage collagens, la 2a 3a, C-PS 1, and C-PS 2, were purified, antibodies raised, and then used in immunofluorescence studies on bovine nasal cartilage (BNC). Punctate This report describes the localisation of these collagens in BNC by immunofluorescence.Materials and methods Collagens. These were isolated from various bovine tissues after limited pepsin digestion. Type II, la 2a 3a, C-PS 1, and C-PS 2 collagens were isolated from adult nasal cartilage as described by Ayad et al.7 8 Types I and III collagen were prepared from placental membranes according to Epstein.' Types IV and V collagen were isolated from placental villi and membrane respectively by the method of Abedin et 575 on 11 May 2018 by guest. Protected by copyright.

A simplified procedure for the fractionation and purification of different collagen types from various tissues is described which is particularly efficient in separating type-V from type-IV collagen, and high-mol.-wt. (HMW) aggregates from 7 S collagen. Uterus and maternal villi contain 2 forms of type-V collagen - [alpha 1(V)]2 alpha 2(V) and [alpha 1(V) alpha 2(V) alpha 3(V) - which have been separated on DEAE-cellulose. Uterus however appears to be the richest source of both HMW aggregates and the [alpha 1(V) alpha 2(V) alpha 3(V)] collagen, and a probable relationship between these collagens is discussed.

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