The present study was designed to identify the constituents of the fixed oil from seeds of Sudanese Cajanus cajan (L), and to evaluate its antimicrobial activity. GC-MS analysis of the fixed oil revealed the presence of 9,12octadecadienoic acid (35.28%), hexadecanoic acid (19.74%), 9-octadecenoic acid (19.14%) , methyl stearate(6.71%) beside other minor constituents. In cup plate agar diffusion assay, the oil was screened for antimicrobial activity against six standard human pathogens and promising results were obtained.

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