Bupleurum scorzonerifolium Willd has been found to have a wide range of immunopharmacologic functions. We isolated an anti-UVB B. scorzonerifolium cell clone and found elevated level of polysaccharides. In this study, we investigated the ability of crude polysaccharide (CP) from the anti-UVB B. scorzonerifolium cell clone to inhibit UVBinduced photodamage using a human skin keratinocyte cell line, HaCaT. Cells were UVB irradiated and then incubated in presence of different concentrations of CP. MTT assay showed that the CP did not induce cytotoxic effect under 10 mg/mL and after UVB irradiation, CP can inhibit UVB-induced HaCaT cell death. Decreased reactive oxygen species and lipid peroxidation and increased superoxide dismutase activity showed that CP can act as a free radical scavenger. Furthermore, CP had a strong protective ability against UVB-induced DNA damage. These effects were compared to the crude polysaccharide (CP 0 ) from normal B. scorzonerifolium callus at concentration of 20 mg/mL. The portion of crude polysaccharide (CP) from the anti-UVB B. scorzonerifolium cell clone was more than 2.5-fold higher than crude polysaccharide (CP 0 ) from normal B. scorzonerifolium callus. Taken together, the protective mechanisms of crude polysaccharide from the anti-UVB B. scorzonerifolium cell clone against UVB-induced photodamage occur by the inhibition of UVB-induced reactive oxygen species production, lipid peroxidation and DNA damage.Keywords Anti-UVB Bupleurum scorzonerifolium cell clone Á Crude polysaccharide Á Oxidative stress Á Photodamage Á UVB

From the learning motivation, training process, professional direction of the diversified characteristics, this paper analyzes the realistic requirements of personalized training of military professional degree students, analyzes the problem of current personalized training of military professional degree students that training courses teaching lack of humanity, courses research lack of pertinence, and the training standard is not clear, put forward the suggests that graduate student selection protruding flexibility strategy, subject based on the problem oriented, study research subject to the forces demand, and cooperative establish the graduate training standards to improve the personalized professional degree graduate training ability.

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