Introduction The prevalence of H. pylori infection in Indonesia (Medan 52, Jakarta 40‐57, Mataram 51‐66%). And Non Serological Diagnostic test for H. pylori, with methods Invasive (CLO, Microbiology and Histopatology) and Non invasive (UBT), but cost this method is expensive for the lower class and rural area. Background H. pylori produces of Urease enzymes and urease activity is an important characteristic of the Organism, so How about Urea and Ammonium concentration in Gastric juice patients H. pylori infection? Objective Gastric juice analyzed for Urea and Ammonium H. pylori Infection, compare with non infection H. pylori, and the future Gastric juice aspiration to analyze by Naso gastric tube ?. Material and methods Patients with chronic dyspepsia, without consumption antibiotics, omeprazole/H2RA within 1 month, no ureami and investigation with upper endoscopi to gastric juice aspiration and CLO test. Gastric juice analyzed by colorimetric method and spectrophotometric assay. Patients control: no symptom dyspepsia, IgG anti HP negative, Upper endoscopy normal. Result Preliminary report H. pylori positive‐patients bad lower urea and higher ammonia concentration compared with non infection H. pylori (control) Conclusion The Third Western Pacific Helicobacter Congress, June 22‐24 Bali‐Indonesia.