Dendrobium heterocarpum Lindl. is a native orchid in Indonesia. Its population continue to decline due to unsustainable exploitation. One solution to increase its population is via in vitro technique propagation. This research aimed to determine effect of hormone addition (Zeatin and NAA) to induce shoot multiplication of D. heterocarpum Lindl. grown in Murashige and Skoog (MS) basic medium. The research was conducted in Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Science, Udayana University from November 2015 until March 2016. The experiment employed Completely Randomized Design (CRD) with 20 combinations of treatments, with three replicates each treatment. The treatment werer addition of Zeatin and NAA, with five concentrations of Zeatin (0, 0.1, 0,5, sweet corn and hybrids corn as the natural Zeatin); four NAA concentrations (0, 0.05, 0.1 and 0.5). Variable recorded include such as the number of shoots, roots and explant’s vigor. The quantitative data which include number of leaves and shoots after 12 weeks, were analyzed using Analysis of Variance (ANOVA); and if significantly different, tested further using Duncan Multiple Range Test (DMRT) at 5% level. Z5N3 medium revealed significantly different on amount of leaves and shoot height and also the best medium on amount of roots. Best hormone combination that can induce multiplication of new shoots are medium Z4N3 and Z5N1.

Keanekaragaman flora menjadi kekayaan hayati bagi kehidupan di alam. Penelitian bertujuan mengeksplorasi jenis dan manfaat tumbuhan di sekitar desa Mincidan agar dapat digunakan sebagai dasar pendidikan dan pengembangan ekowisata.   Metode deskriptif kualitatif dengan survei dan wawancara dengan penduduk desa dilakukan untuk melengkapi data yang dapat dikembangkan dalam rencana konservasi tanaman.  Ragam tanaman yang dijumpai di sepanjang rencana jalur trekking sepanjang 1,5 km tergolong pada tanaman obat, hias, buah, dan upakara.   Berdasarkan hasil survey, dapat diidentifikasi 102 jenis tumbuhan, 71 genera yang termasuk dalam 38 familia. Tanaman tersebut dapat dimanfaatkan untuk bahan upacara keagamaan dan obat tradisional.

The aim of the research was to create genetic variation on garden balsam using oryzalin and Gamma radiation. Mutagenic agent may improve plant variation. Garden balsam seeds were treated by oryzalin (0%, 0.01%, 0.02%) then the seedlings were planted in the field four times to gain seeds generation M4. Seedlings of M4 (7 days of age) were radiated by gamma ray 60Co (0, 5, 10, 15 Gy) and planted in the field to collect seeds for in vitro study to induce a new type of mutant plant. In vitro methods was conducted to achieved rapid micropropagation of Impatiens balsamina L. mutant plants. Growth percentage of seedlings reached 90% by gibberellin 0.01 ppm. Leaf section used for explant and cultured in MS media enriched by 0.5 ppm NAA and 0.5 ppm BAP aseptically. Shoots were regenerated on 6 weeks after cultured however some shoots become vitrification. Number of chromosome varied (mixoploid) on treated plants (M5). Form of secondary metabolites ( alkaloids and terpenoids ) in the roots extract was not changed by oryzalin and gamma radiation. Mixoploid explants showed variation in morphology and some treatments only had little shoots and a treatment has vigorous roots. Control plants have shoot and callus.

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Arenga palm (Arenga pinnata) is one species from palmae family.   Economically, the palm is suitable for home industry, such as Balinese offering which use parts of young leaf, sap can be used as special local genius sweet drink from Bali, fruits are boilled that can be mixed with sugar for healthy ice.  Arenga palm  can be propagated aseptically using tissue culture technique.  Culture media may varried between MS and WPM with or without adding hormone.   Auxin can be added as 2.4-D ( 4 ppm) to induce callus.  NAA and BAP with concentration 1.5 ppm respectively also added to induce multiplication of shoots or roots.      Each treatment had 10 replicatations.  Six weeks after cultured, callus growth observed only at WPM media that enriched with 2,4-D.  The others treatment show the explant was dormant because did not show any growth while analysed anatomically under microscope.