Semen of ayu Plecoglossus altiveris altiveris cryopreserved with a diluent comprising 10 MeOH(cryoprotectant) and 90 fetal bovine serum (FBS: extender) showed a higher rate of motility than that cryopreserved with other cryoprotectants (DMSO, glycerol, N,N dimethylacetamide, N,N dimethylformamide, ethanol) and extenders (ayu serum, artiˆcial seminal plasma, 300 mM or 600 mM glucose).
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