Root zone soil moisture (RZSM) is an essential variable for weather and hydrological prediction models. Satellite-based microwave observations have been frequently utilized for the estimation of surface soil moisture (SSM) at various spatio-temporal resolutions. Moreover, previous studies have shown that satellite-based SSM products, coupled with the soil moisture analytical relationship (SMAR) can estimate RZSM variations. However, satellite-based SSM products are of low-resolution, rendering the application of the above-mentioned approach for local and pointwise applications problematic. This study initially attempted to estimate SSM at a finer resolution (1 km) using a downscaling technique based on a linear equation between AMSR2 SM data (25 km) with three MODIS parameters (NDVI, LST, and Albedo); then used the downscaled SSM in the SMAR model to monitor the RZSM for Rafsanjan Plain (RP), Iran. The performance of the proposed method was evaluated by measuring the soil moisture profile at ten stations in RP. The results of this study revealed that the downscaled AMSR2 SM data had a higher accuracy in relation to the ground-based SSM data in terms of MAE (↓0.021), RMSE (↓0.02), and R (↑0.199) metrics. Moreover, the SMAR model was run using three different SSM input data with different spatial resolution: (a) ground-based SSM, (b) conventional AMSR2, and (c) downscaled AMSR2 products. The results showed that while the SMAR model itself was capable of estimating RZSM from the variation of ground-based SSM data, its performance increased when using downscaled SSM data suggesting the potential benefits of proposed method in different hydrological applications.