Abstract:The study was aimed at standardization of cryopreservation protocol for the common carp, (Cyprinus carpio) spermatozoa for using in the artificial propagation. Three extenders (Alsever's solution, egg-yolk citrate and 0.9% NaCl) were combined with three cryoprotectants (ethanol, methanol and DMSO). Two-step freezing protocol (5 o C to -4°C at a rate of 4°C per minute then -4°C to -80°C at a rate of 10°C per minute and finally held for 10 min) was carried out in a computer-controlled freezer where 0.25 ml straws containing spermatozoa were stored in a liquid nitrogen container at -196 o C. Alsever's solution with ethanol, Alsever's solution with methanol and egg yolk citrate with DMSO gave better motility (%) in both pre-freeze (93.33±1.05, 90±1.83 and 88.33±2.11, respectively) and post-thaw (80±4.28, 74.17±3.96 and 76.67±4.59, respectively) periods whereas, 10% cryoprotectant gave the best motility during post-thaw period. Considering both pre-freeze and post-thaw motilities of the common carp spermatozoa, 1:9 was the best ratio for the milt and cryodiluents. The egg yolk citrate with DMSO yielded the best fertilization (37.67±1.76%) and hatching (28.67±1.85%) compared to other combinations under study.

The physiological changes in the thyroid gland during pregnancy were well understood. Serum TSH levels were significantly increased without changes in free T3 and T4 in preeclamptic patient compared to normal pregnancy. This study tried to find out a possible relationship between preeclampsia and thyroid profile. The present study determines thyroid hormonal levels in cases of preeclampsia in the third trimester of pregnancy. TSH level were significantly higher in preeclamptic group as compared to control. A prospective case control study was conducted in the Department of Obstetrics and Gynecology, Chittagong Medical College Hospital, Chittagong. Thyroid hormone, namely Triiodothyronine (Free T3), Thyroxine (Free T4) and Thyroid Stimulating Hormone (TSH) were evaluated by radioimmunoassay. In this case control study pregnant women with preeclampsia were recruited after the diagnosis were made and compared with equal number of healthy normotensive pregnant women in third trimester. The data were analyzed using unpaired ‘t’ test and chi-square test. When P value were <0.05, the result were accepted as significant. Increase TSH level were associated with a risk for occurrence of preeclampsia DOI: http://dx.doi.org/10.3329/medtoday.v25i2.17922 Medicine Today 2013 Vol.25(2): 63-66

This study was designed to determine progress of women presenting with dysfunctional uterine bleeding (i.e

Background: Unhealthy cervix is a very common finding in Bangladesh due to poor genital hygiene, malnutrition, and multiparity. Cervical Cancer is a serious health problem in Bangladesh and other developing countries. Cervical Cancer is a preventable cancer due to long preinvasive state and available cervical screening program like pap smear, colposcopy etc. Upon detection the treatment of pre-invasive lesions is highly effective. The objective of our study is to observe and evaluate suspicious cervix upon specimen collection by pap smear and viewing them by colposcopy when required.   Materials and methods: This is a descriptive type of observational cross-sectional study conducted in the Department of Obstetrics and Gynecology of BBMH, Chittagong over a period of six months from December 2020 to June 2021. A total of 64 women were selected, who came to hospital outdoor for the treatment of persistent vaginal discharge, post coital bleeding, postmenopausal bleeding. A thorough general, systemic and pelvic examination was done. Pap smear was conducted and colposcopy was done for women who had persistent inflammation on pap smear cases. Multiple variables like age of marriage and first intercourse, parity contraception was taken in consideration. The data obtained were recorded and statistically analyzed.                        Results: Out of 64 women the minimum age was 30 and maximum was 62 years with a mean age of 32.6 ± 7.2 years. Of the 64 women with unhealthy, inflammatory pap smear cases, 33 (51.6%) patients were colposcopically positive for CIN. Then histopathological evaluation of the biopsy material of those 33 women were done. It revealed 12 (36.3%) with abnormal cytology. Patients with positive CIN experienced early marriage, early age of child birth, and also had higher parities than patients without CIN. Conclusion: This study should that cervical lesion is one of the commonest problems among the females worldwide, including Bangladesh. This revealed the importance of emphasizing on proper screening of carcinoma cervix to decline incidence and mortality in developed and developing countries. JCMCTA 2022 ; 33 (1) : 71-76

To determine the effectiveness of Mirena in achieving a reduction in heavy menstrual blood loss in dysfunctional uterine bleeding. To evaluate improvement in Haemoglobin level (Hb gm%) in patients during treatment period. The study was conducted in the department of Obstetrics & Gynaecology, Chittagong Medical College Hospital, Chittagong, Bangladesh from August 2008 to July 2010. Thirty six patients with Dysfunctional Uterine Bleeding (DUB) in 30-45 years age group with Body Mass Index (BMI) of 18-30 during treatment period were included in this study. They were counseled, investigated and Levonogestrel Intrauterine device Mirena was inserted. Outcome was measured by the Hb improvement and decrease in blood loss both in amount and duration. Record was kept and follow up was done. Ninety percent of the patients were relived from menorrhagia and one hundred percent improvement was seen in cases with polymenorrhagia. Fifty percent patients had an Hb of >11.1 gm% after 3 months of use. Mirena is a safe and effective device for treatment of DUB patients and it is an alternative to hysterectomy. JCMCTA 2014 ; 25 (1): 8-11