This study was conducted to investigate the protective effect (at level 7.5% of marrow vegetable, lettuce seed, prickly pear seeds) and mixture of them at 1:1:1ratio on liver and kidney functions, blood lipid profiles and blood sugar in rats fed with diets contain height level of cholesterol. Thirty mature albino male rats weighing 150 + 5 g. B.WT. each, were divided into sex equal groups, The first group kept as a control-ve group, while the others five groups fed on diet containing high level of cholesterol for 3 weeks to obtain hypercholesterolemic rats. At the end of experimental period (28 days), animals were sacrificed. Blood samples were collected to determine the biochemical analytics: serum total cholesterol (T.C.), triglycerides (T.G), lipoprotein fractions (HDL-c, LDL-c, and VLDL-c), atherosclerotic index (AI), liver enzymes (AST, ALT and ALP), total bilirubin, urea, uric acid, creatinine and fasting plasma glucose. Moreover, heart, liver and kidneys subjected to for histopathological examination. The obtained results concluded that diets of marrow, lettuce, prickly pear seeds and their mixture caused the improvement of all biochemical analysis.