Recombinant adenoviruses are being developed for gene virus containing the E. coli lacZ reporter gene (Ad2␤gal2) therapy for cystic fibrosis and other lung diseases, and for in the presence of various polyions. For each cell type, prevention and treatment of vascular thrombosis. A major adsorption of Ad2␤gal2 in the presence of the polycations limitation to the clinical utility of adenoviruses is the low polybrene, protamine, DEAE-dextran, and poly-L-lysine efficiency of gene transfer achieved in vivo. In addition, significantly increased the percentage of cells that express little is known about the initial interactions between adenolacZ. The polyanion heparin did not significantly alter gene viruses and the target cell. To address the hypothesis that transfer efficiency, but completely abrogated the effects of the negative charge presented by membrane glycoproteins polycations. These data provide evidence that negatively reduces the efficiency of adenovirus-mediated gene transcharged moieties on the cell surface reduce the efficiency fer, primary cultures of human airway, Madin-Darby canine of adenovirus-mediated gene transfer, and that alteration kidney cells, an immortalized cystic fibrosis airway epiof the charge interaction between adenoviruses and the thelial cell line, and primary cultures of sheep pulmonary cell surface may improve the potential clinical application artery endothelium were infected with recombinant adenoof these vectors.

Many caregivers of children with T1D or CF experience depressive symptoms, although risk factors may differ in these two populations. Screening of caregiver depressive symptoms as part of routine clinic visits may provide opportunities for needed intervention.