BACKGROUND: Essential oils are reported to be bio-insecticides. However, problems related to their volatility, oxidation and poor water solubility need to be solved before they can be considered as replacement pest control methods. Thus, an appropriate formulation is needed for commercial use. In this work, a new control method based on the use of a powdered cyclodextrin (CD)/1,8-cineole inclusion complex was assessed against larvae and adults of Ectomyelois ceratoniae (Zeller) in comparison with two Rosmarinus officinalis (L.) essential oils, free 1,8-cineole (oils major compound) and a mixture of 50% CD/1,8-cineole inclusion complex and 50% free 1,8-cineole. −1 air, the respective mortalities were 94.12%, 35.29% and 19.61% for solid CD/1,8-cineole complex, Thala and Cap Zbib oils after 37 days of exposure. Moreover, the solid CD/1,8-cineole complex achieved 5% adult emergence versus 68.33% for Cap Zbib and 55% for Thala oils. The half-life of solid CD/1,8-cineole inclusion complex (10.98 days) was considerably longer than that of the mixture (7.53 days) or free 1,8-cineole (3.43 days). CONCLUSION: This work highlights the utility of essential oil encapsulation in CD to overcome restrictions when used to control the date moth E. ceratoniae during storage.
RESULTS: Solid CD/1,8-cineole complex was more toxic against E. ceratoniae larvae than the two crude essential oils. At a concentration of 15 L liter
Chemical composition of Algerian Mentha rotundifolia and Myrtus communis essential oils, their insecticidal activities and their impact on some biological parameters of the Mediterranean flour moth Ephestia kuehniella were assessed. Results showed that M. rotundifolia essential oil contained piperitenone oxide (46.06%), d-limonene (9.10%), cis-piperitone oxide (6.81%), and endo-borneol (4.64%) as major compounds, while M. communis oil was rich in α-pinene (29.08%), 1,8-cineole (36.82%), α-terpineol (6.42%), geranyl acetate (4.38%), and β-linalool (4.04%). The fumigant potential and contact toxicity tests against E. kuehniella demonstrated the effectiveness of M. rotundifolia essential oil (LC50 = 0.54 μL/L air, LC50 = 0.004 μL/cm2) compared to M. communis oil (LC50 = 2.91 μL/L air, LC50 = 0.025 μL/cm2). Moreover, results revealed that all biological parameters were significantly affected (fecundity: 6 eggs/female, oviposition deterrence: 96.62%, log fertility: 0, hatching rate: 0%, copulation rate: 0% for M. rotundifolia oil against fecundity: 93 eggs/female, percentage of oviposition deterrence: 47.85%, log fertility: 6.7, hatching rate: 57%, copulation rate: 53.33% for M. communis oil). This work supports the use of botanical insecticide as active pest control agents under storage conditions.
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