T wo fIelD experiments were carried out on clay soil in el-Gemmeiza Agric. Res. St., ARC, el-Gharbiya Governorate, during 2016 and 2017 to study the effect of nanofertilizer and N-fertilization levels on productivity of cotton Giza 86 under two sowing dates. The experiment design was a split split-plot. The main plots involved two sowing dates; (early at 8 th April and late at 8 th May). While, the sub plots were allocated to three treatment of nanofertilizer (without, lithovit 2.5g/l and lithovit 5g/l). Application three times (at squaring, initiation of flowering and two weeks after flowering). While, the sub sub-plots were allocated to three N-fertilization levels, (50%, 75% and 100% recommended dose). The most important results obtained could be summarized as follows: Sowing date had a significant effect on growth, yield and its components. Where, the early sowing date surpassed the late sowing date. The levels of N had significant effect on growth, seed cotton yield and its components in both seasons. The rate of N (75%) gave the good averages in this respect. The Nano-fertilizer by Lithovit had significant effect on growth, seed cotton yield and it compounds. All treatments had a not significant effect on fiber properties. Early sowing date in combination with the N fertilizer (45kg N/fad) and foliar application with Lithovit (5g/L water) for obtaining gave the high productivity of egyptian cotton variety Giza 86.