Water-distilled essential oil from the leaves of Thecocarpus meifolious Boiss. which is endemic to Iran, collected from the province of Chaharmahal-Bakhtiyari, west of Iran, was analyzed by GC and GC-MS. The leaf oil of T. meifolious investigated to analyze their antimicrobial activity, antioxidant and total phenolic content. Antioxidative properties of this oil was determined by 3 methods i.e., by ferric-reducing antioxidant power (FRAP), radical-scavenging capacity of the oil or bleaching of 2,20-diphenylpicrylhydrazyl (DPPH) and β-carotene-linoleic acid assay. Spathulenol (20.51 %), camphor (10.20 %) and caryophyllene oxide (10.35 %) were the major components in this oil. This oil indicated significant activity Candida albicans and moderate inhibitory activity against the gram-negative bacteria. The results suggest application of T. meifolious oil as a natural antioxidant agent.