This study was carried out to evaluate the relationship between narghile smoking and serum concentration of serotonin and endorphin and some blood hematological indices in males. Group of smokers composed of 50 voluntary, and control group composed of 36 healthy voluntary. This study was conducted on adults man that ages between (18-25 years). The markers measured in this study were serum serotonin and endorphin and hematological indices (platelets, hematocrit, hemoglobin, and white blood cells). This study showed a significant decrease (P<0.05) in levels of serum serotonin, endorphin and blood platelets, and a significant increase (P<0.05) in levels of in blood hematocrit and hemoglobin and a non-significant increase of white blood cells (P>0.05) in smokers as compared with healthy persons.