No abstract

The aim of present investigation was to study the antibacterial activity of raw honey of three bee species collected from Kannad taluka of Aurangabad district. Honey samples were collected and assayed against four bacterial species by using Agar well diffusion method. All honey samples showed sensitivity against four bacterial species. Zone of inhibition clearly showed that honey obtained from Apis florea and Apis cerana indica was highest antibacterial activity, while honey of Apis dorsata was minimum antibacterial activity. Antibacterial activity showed that Bacillus subtilis was more sensitive than Salmonella typhi, Escherichia coli and Staphylococcus aureus.

The present study deals with the effect of storage on Apis mellifera honey quality parameters harvested from Kannad taluka of Aurangabad district (M.S.), India. Honey quality parameters like pH, electrical conductivity, moisture content, proline content, HMF content, invertase and diastase activity were measured by AOAC method (2000) at intervals of each six month for the period of 24 month. Student's T-Test was applied to the analyzed data. Results clearly indicates that the pH, electrical conductivity, moisture content and HMF content were increased with the increasing of storage period and concentration of proline, invertase and diastase number were decreased with the increasing of storage time limit. The said physicochemical parameters of honey sample were found statistically significant according to their storage time limit. The obtained data also clearly demonstrate that the invertase is more sensitive than the diastase and HMF content to the storage as well as heat treatment of honey.

The aim of present investigation was to study the antifungal activity of raw honey of three honeybee species collected from Kannad taluka of Aurangabad district. Honey samples were collected and assayed against three fungal species by using Agar well diffusion method. All honey samples showed sensitivity against all the fungal species. The zone of inhibition clearly showed that honey obtained from Apis florea and Apis cerana indica having highest antifungal activity, while honey sample of Apis dorsata showed minimum antifungal activity. The most sensitive fungi were Aspergillus and Fusarium, while Alternaria spp. was less sensitive to the inhibitory action of honey. Keywords: Honey, Apis dorsata, Apis florea, Apis cerana indica, zone of inhibition, antifungal activity, sensitivity, etc.

The present study deals with the enzyme activity of honey samples harvested from three different locations of Kannad region of Aurangabad district (M. S.), India. Diastase and invertase activity were analyzed by using Schade et al., (1958) and Siegenthaler (1977) method respectively. Results clearly indicate that the honey harvested from agricultural and forest area shows highest enzyme activity than road side area. The values of enzymes in honey were varied from location to location. This study also clearly demonstrates thin viat honey harvested from Kannad region were fresh and unheated because the honey samples have more enzyme activity. The values of diastase and invertase were within the quality regulation limits proposed by Codex standards.