Aim: Formation of niosome by using non-ionic surfactant. The particle size of niosome must be required in the range in between 10 nm-100 nm. This is just due to avoid the aggregation of niosome and show proper result. Materials and Methods: There are many types of niosome, their types and size depend on which method used for preparation. In this article we covered method of preparation of pro-niosome and niosome. Many factors are affecting formation of niosome such as drugs, its chemical and physical property, amount and type of surfactant, cholesterol content and its charge, resistance to osmatic stress as well as membrane composition. Various method is used for separation of unentrapped drug from final product such as dialysis, gel filtration and centrifugation. There are several routes used for administration of niosome such as oral, parenteral, transdermal and ocular etc. Results: The evaluation and characterization of niosome done by many methods such as entrapment efficacy, vesicle diameter, in-vitro release and loading efficiency. Niosome have many pharmaceutical and non-pharmaceutical applications; which having marketed product of niosome are available in market are explain in this article. Conclusion: Niosome formation methods are completely based on liposome methods of preparation. Niosome are having more storage capacity than liposome i.e. niosome are more advance than liposome. The cost of preparation of niosome is also less than liposome. Many pharmaceutical formulations of niosome are now available in market.

Liposomes are the most advance formulation for targeting and controlled drug delivery system. These liposomes are generally administered by intra-venous route. In this work the liposome was prepared by using thin film hydration method. The formulated liposome is evaluated or characterised by using zeta sizer, Encapsulation efficiency, Entrapment efficiency, In vitro drug release. Main things are drug which are used for formulation of liposome was Diclofenac sodium, it having anti-inflammatory and anti-pyretic effect. The Diclofenac sodium having several adverse effects, such as depression of renal function, Liver failure for repeated administration, Local mucosal irritation, gastritis. To avoid this adverse effect Diclofenac sodium are incorporate in liposomal formulation. By formulating liposomal formulation, the bioavailability of Diclofenac sodium increase. In conventional dosage form bioavailability of diclofenac sodium is 50℅. But in liposomal formulation bioavailability of this drug increase. The final result includes that diclofenac liposome formulation shows more sustained and prolong anti-inflammatory activity. Keywords: Diclofenac sodium, Liposome, Anti-inflammatory activity.

Acyclovir is also known as Acyclovir, this drug is used for treatment of viral infection, particularly for treatment of herpes simplex viral infection. These are taken for month during treatment of herpes simplex viral infection. These are showing the action against all herps virus family. The acyclovir is poorly water-soluble drug. Due to that main aim is to increase the solubility of acyclovir in other solvent. The bioavailability of acyclovir is very less about (15-35%) because it has less oral route absorption. Due to that the acyclovir are given in intravenous route. When acyclovir is taken in oral route, the peak plasma concentration occurs after 1-2 h. The acyclovir having 9-33% of plasma protein binding. The BCS class of acyclovir are Class third (high solubility and Low permeability). Due to that acyclovir are formulate in the form of nanoparticle. Chitosan are the polymers which are used for the formulation of nanoparticle. The chitosan is found to be compatible with acyclovir. Formulation of acyclovir nanoparticle was done by Nano-precipitation method. Many evaluation tests performed during the formulation of Acyclovir nanoparticle mainly zeta sizer is use for the determination of particle size, zeta potential and PDI (poly disperse index) also performed evaluation of loading efficiency and % Drug entrapment. Keywords:  Acyclovir, Chitosan, Zeta sizer and Nanoparticle.