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The aim of the data were to increase the treatment efficiency of pyrene from soil using Nano catalysts magnetite iron oxide (Fe 3 O 4 ) and combined with electro kinetic. Soil provided with 100 mg/kg concentration and removal of pyrene done with EK-Fenton process. Nano catalyst was synthesized via a facile co-precipitation method and characterized by FTIR, XRD, SEM, EDX, VSM techniques. The effects of some operational parameters include catalyst dosage, pH, hydrogen peroxide concentration and the voltage were studied on the removal efficiency of pyrene. Results indicated the removal efficiency was obtained 87% under optimal conditions (pH = 3, Nano catalyst dosage= 1 g/l, H 2 O 2 =10 mM and voltage 30 V). Electrokinetic Fenton process can be as efficient and effective method for the removal of pyrene from contaminated soil using Nano Catalyst Fe 3 O 4 introduced in optimal conditions.