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, the sequences that rnake a perfect match only with their complernentary strands. USPs were setected by a program "T-GUSP" which we wrote in C. T-GUSP mainly consisted ef two modulcs, thc i'lrst medule select the set of USPs by ovcrlapping adcquatc number of Seeds (typically 7 nucleotides in lcngth) and the second assign the USPs according to the design. The set of VSPs weTe evaluated by the free energy using dynamic programingalgorithmincorporatedintoT-GUSP.Allstructureswereassembled by one pot annealing where equivalent molecule ofshort and long strands werc mixed in annealing bufler and cooled from 96 deg. to room temperature over the course of24 hours. The successfu1 buiiding ofstructures was confirmed by gel electrophoresis and thermal rnelting profilcs, 3J0936 Purification of ferritin "'ith magnetic nanoparticlcs YusukeHikichi,TornoakiHaTada,HirokoFukano,HideyikiYoshimura(Mblii U}tiv.)Apofenitin is a spherical protein with a diameter of 1 3 nm and intemal cavity of 7 nm, in which the protein forrns a t'errihydrite core. Apoferritill is also known to tbrm cores of other metal compounds in thc cavity such as cobalt, nickcl, or chromiuminvitro.Herewereportsynthesisofmagnetite(Fe304)nanopart{c1es in thc apo-fenitin cavity and separation methodology of apot'erritin and apoferritin with magnetite cores.

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