Ximenia americana is endemic in Northern Nigeria, and has been reported to be used in treatment of various types of ailments. In the present study, the antimicrobial properties of the stem bark extracts of Ximenia americana were screened against Escherichia coli, P.aeruginosa, Staphylococcus aureus, P. vulgaris, Candida albicans, B. subtilis using the disc diffusion method. The result revealed the methanolic and water extract showed significant (P<0.05) broad spectrum activity on the growth of many of the test organisms (E. coli, and P. vulgaris, S.aureus, P. aeruginosa and B. subtilis), while the butanolic extract had little activity. Phytochemical screening revealed the presence of alkaloids, saponins, flavonoids, cardiac glycosides, terpenoids and tannins. The study supports the traditional usage of this plant by herbalist as remedy in curing microbial infections.