No abstract

Many studies have associated the consumption of (poly)­phenol-rich diets with health benefits. However, accurate high-throughput quantitative methods for estimating exposure covering a broad spectrum of (poly)­phenols are lacking. We have developed and validated a high-throughput method for the simultaneous quantification of 119 (poly)­phenol metabolites in plasma and urine using ultra high-performance liquid chromatography coupled with triple quadrupole mass spectrometry, with a very fast sample treatment and a single run time of 16 min. This method is highly sensitive, precise, accurate, and shows good linearity for all compounds (R 2 > 0.992). This novel method will allow a quantitative assessment of habitual (poly)­phenol intake in large epidemiological studies as well as clinical studies investigating the health benefits of dietary (poly)­phenols.

Heat treatment exerts a positive effect on the bioaccessibility of artichoke (poly)phenols after gastrointestinal digestion. In the first 2 h of fermentation, native (poly)phenols were readily degraded by an important microbial catabolic activity.