Nursing practice environment excellence is crucial for the enhancement of health care system performance.Nurses need a work environment that makes them use the full expression of their skills and knowledge. The aim of this study was to assess the perception of nurses' work environment in tertiary care hospital in Saudi Arabia. A descriptive cross-sectional correlation research design and a convenient sampling technique was used to recruit 541 bedside nurses and nursing administrators working in the hospital with a response rate of 98.5%. A self-report questionnaire consists of demographic Characteristics and the Nursing Practice Environment scale. The results indicated that the mean score of nurse participants in hospital affairs was 3.0 (SD 0.47), nursing foundation for quality of care was 3.1 (SD 0.45), nurse manager ability, leadership and support of nurse was 2.9 (SD 0.60), staffing and resources adequacy was 2.6 (SD 0.70) while the mean score of collegial nurse-physician relations was 2.9 (SD 0.57). The overall mean score of the Nursing Practice Environment scale was 2.9 (SD 0.47). This study can guide policy makers, administrators, nurse leaders and educators, to identify areas to improve nurses' work environment; which could translate into significant improvement in patient outcomes.