A total of 75 one-day old chicks (Cobb local breed) At the 1 day old the chicks were randomly divided into 3 equal separate groups; 25 chicks each. Group 1 fed on prebiotic beta glucan 200gram/ton, group 2 fed on probiotic saccharomyces servicee 500gram/ton and group 3 control fed on balanced commercial ration. The result revealed that the group of broiler fed on prebiotic increase the lysozyme more than the group of broiler fed on probiotic and after artificial infection, the group treated with prebiotic was able to face the clostridial infection more than group treated with probiotic. The histopathological examination showed that increase length and width of intestinal villi of chicken treated with prebiotic more than that of probiotic.

Ceacal coccidiosis one of Nine Eimeria species affecting chickens and results in severe economic losses. Sixty-seven GIT sample were collected from broiler flocks showing bloody diarrhea in Kalyoubia governorate. 88% of collected samples were found to be positive for cecal coccidiosis after using microscopical and molecular identification. Different control strategies as diclazuril, vaccination (coccivac B ® and isolated strain as field strain vaccine) and the aqueous extract of neem plant (Azadirachta indica) were used to control cecal coccidiosis in experimentally infected broiler chicks. The results showed that the diclazuril was the best protection method as there was a significant improvement in performance parameters, significant drop in oocyst shedding, dropping scoring, lesion scoring and cecal mucosal scraping scoring. Also, there was minimal histopathological alteration in the cecum of infected broilers in comparison with other treatments. The neem extract treated group also recorded an improvement in the aforementioned parameters in comparison with the vaccinated infected birds. The vaccination of birds using isolated strain achieved better protection against coccidia infection than the imported strain.

A total 100 samples of liver and intestine from broilers (apparently diseased) from 13 poultry farms were analyzed to detect the incidence of C. perfringes. The samples were examined bacteriologically by cultivating on CMC and blood agar, typical colonies were identified and biochemically confirmed. The total number of positive samples was 46 (46%). C. perfringens showed very high sensitivity to enrofloxacin, high sensitivity to amoxicillin and cefoperazone, but resistant for mupirocin and azteronam. By making artificial infection for mice, the mice died after 24 hours and by examination histopathologicaly, it showed liver degeneration and desquamation of epithelial cells of intestine. Multiplex polymerase chain reaction method (PCR) was amplified for toxin genotyping by using primer for clostridium perfringes alpha toxin which amplified at 402 bp.