Aimed at the human comfort problem with cantilevered floors arising from normal human activities, the Tuned Mass Dampers are used to control the vibration of the steel cantilevered floors which span 16.3m. Based on studies on the dynamic characteristics of the overall structure, with the position of TMDs optimized and the parameters set reasonably, the vertical vibration response of the cantilevered floors under different cases of pedestrian walking loads is calculated. The results show that the TMD system can effectively reduce the dynamic response of the cantilevered floors, in order to meet the requirements of human comfort.