Pigments are widely distributed in sugarcane plant cells and have been associated with color in sugarcane juice for white sugar production. Chlorophylls, phenolics and flavonoids compounds are some of those pigments. Mechanized harvesting increases the adding of cane tops to processing and the study of these compounds allows us to understand their impact on the final product. The aim was to separate and quantify these compounds obtained from sugarcane top ethanolic extracts. The chromatographic occurred in adsorption resin. The quantification was made in spectrophotometric systems. These methods allow separation and quantification of compounds and even observation of polarities of molecules compared to the eluents.