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Lack of degradability and the closing of landfill sites as well as growing water and land pollution problems have led to concern about plastics. With the too much use of plastics and increasing pressure being placed on capacity available for plastic waste disposal, the need for biodegradable plastics and biodegradation of plastic wastes has assumed increasing importance in the last few years. Awareness of the waste problem and its impact on the environment has awakened new interest in the area of degradable polymers. The interest in environmental issues is growing and there are increasing demands to develop material which do not burden the environment significantly. This project reviews the biodegradation of biodegradable and also the conventional synthetic plastics, types of biodegradations of biodegradable polymers also use of a variety of “Recent development of biodegradation techniques” for the analysis of degradation in vitro.

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A collagenase from Pseudomonas sp. was purified upto 13-fold with a yield of 3.6% using hydrophobic interaction chromatography. Molecular weight of 34kDa collagenase was identified using sodium dodecyl sulfate-polyacrylamide gel electrophoresis method. Gelatin and collagen substrates were used in the characterization of purified collagenase. The optimum temperature and pH were 45 °C, pH 7.5 for gelatin and 50 °C, pH 8.5 for collagen respectively. The collagenase activity was highly inhibited by Hg2+, Pb2+, EDTA, DTT at concentration of 1mM and significant negative effect of Fe2+, Cu2+ and Co2+ was observed at similar concentration. However, Ca2+ (1mM) and PEG (1mM) increased the enzyme activity. The Pseudomonas based collagenase have the ability to hydrolyse protein substrates. The K m and V max values for the purified collagenase were found to be 1.65mg/mL and 2.12U/mL with collagen substrate while for gelatin substrate values were 6.60mg/mL and 6.58U/mL, respectively.

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