Shortage of sugar beet germlasm resources results in the lag in researches of breeding and molecular biology. It is necessary to analyze the major varieties from three major production regions of China. Eighty-eight primer pairs were used to amplify the genomic DNA from leaves of four types of sugar beet varieties with different economic traits, which contain high yield type; high yield, low sugar and Rhizomania resistant type; standard type; medium yield, high sugar and anti-brown spot type, and 33 of which were obtained to be with availability. Two hundred and forty-one varieties from three major regions of China and nine varieties from abroad were detected with 33 primer pairs of SRAP markers. A total of 719 unambiguous bands were obtained, 459 of which were polymorphic. The average ratio of polymorphic bands was 63.8%. Compute over-all mean showed that genetic distance was 0.4165, genetic similarity among varieties was 0.6593, the genetic similarities were 0.7528 among foreign varieties, 0.6945 among monogerm varieties, 0.6816 among polygerm tetraploid varieties, and 0.6612 among polygerm diploid varieties. A total of 250 varieties were divided into four cluster groups based on cluster analysis by MEGA3.1 (at intercept of 0.2). Each genepool from three major regions of sugar beet production in China showed high level of genetic diversity, of which the Northeast genepool showed the highest. The varieties from China and abroad were classified into two different groups by POPGEN32. This indicated definite difference in the genetic background between foreign and native varieties.