This study was undertaken to assess the occurrence of Klebsiella pneumoniae and there in-vitro susceptibility among cattle traders, herdsmen and butchers in Maiduguri cattle market, Borno state, Nigeria. Two hundred and twelve sample (212) sputum samples were collected using wide mouth sterile universal container and transported immediately to Medical Micro-Biology laboratory department, Centre of Excellence, university of Maiduguri teaching hospital (U.M.T.H.) for Laboratory diagnosed. The Sputum samples were culture on MacConkey and Blood agar media and isolated then, identified using Biochemical test which include indole, citrate utilization and urease tests, but only 15 sputum samples were found infected or positive to Klebsiella pneumoniae. And their Antimicrobial susceptibility of the Klebsiella pneumoniae isolate by disc diffusion method shows that Klebsiella pneumoniae are susceptible to these Antimicrobial Drugs: Pefloxacin (93.3%), Tarivid (100%), Sparfloxacin (80%), Ciprofloxacin (93.3%) and on the other hand  Klebsiella pneumoniae are resistance to these Antimicobial drugs; Streptomycin (73.3%), Septrin (80%), Augumentin (73.3%), Gentamycin (66.7%), and Amoxacillin (60%). In conclusion, recommendation have been made on how to curtail the spread of infection caused by Klebsiella in the environment, homes, and between patients. Healthcare providers are advice to follow the specific infection-control precautions.

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