The correction of wall abdominal defects often requires the use of implants such as polypropylene meshes. In spite of presenting good tissue acceptance, these biomaterials can migrate to adjacent viscera, promote enterocutaneos fistulas, tissue adherence and visceral erosions. In this work, the barrier effect of chitosan films associated with polypropylene meshes on adhesion formation experimentally induced in Wistar rats was evaluated. The animals were divided into two groups with 10 animals each. Animals in the CPP group were implanted with chitosan films associated with polypropylene meshes, whereas the ones in the PP group received only polypropylene meshes. After 8 days, the animals were submitted to euthanasia using CO(2) and a descriptive study focusing adhesion formation, visceral involvement with sutures and mesh peritonization was performed. Also, subimplanted material was collected for histopathology analysis. The results showed that the CPP group presented weak adhesions to the omentum over the stitch knots in eight animals. In all animals, the meshes were peritonized, not allowing their visualization after removing the chitosan films. In the PP group, six animals presented intestinal adhesions to the meshes and, in one of them, hepatic adhesion to the mesh was observed, besides omentum adhesion on more than 50% of the mesh area. The protective effect of chitosan films when sutured over polypropylene meshes, as well as no exacerbation of inflammation associated to the peritoneal lesions was statistically demonstrated. Therefore, chitosan films can indeed minimize the formation of peritoneal adhesions induced by polypropylene meshes in rats.

RESUMOO melanoma oral um dos tipos de câncer de pior prognóstico, com alta incidência de metástases. Raças como Cocker Spaniel, Boxer, Golden Retriever, Setter Irlandês são bastante acometidas. O tratamento preconizado é a maxilectomia parcial mas nem todos os proprietários concordam com tal procedimento. Assim, o tratamento alternativo com a criocirurgia tem sido usado, com resultados encorajadores. O presente trabalho relata o caso de um animal portador de melanoma oral tratado com criocirurgia com resultado satisfatório no que diz respeito à melhoria da sua qualidade de vida. ABSTRACTOral melanoma is one of the types of cancer with the worst prognosis and with a high incidence of metastasis. The Cocker Spaniel, Boxer, Golden Retriever and Irish Setter breeds are the first victims of this disease. The recommended treatment is partial maxilectomia but not all woners agree to this procedure. Hence, an alternative treatment with criosurgery has been used, with encouraging results. This study describes the case of an animal with oral melanoma that was treated with cryosurgery, which resulted in an improved quality of life.

Purpose: To verify if the composit poli (2-hydroxyethyl methacrylate)-PolyHEMA/polypropylene mesh implanted in the female rat's abdominal wall could be suitable for the prevention of peritoneal adhesions, and for the evaluation of the tecidual response produced by this biomaterial. Methods: Polypropylene meshes (Group PP, n=20) and polypropylene meshes coated with a layer of poli (2-hydroxyethyl methacrylate)-PolyHEMA (Group PH, n=20) were implanted on the abdominal wall of Wistar female rats. Ten animals from each group were submitted to euthanasia at 15 and 30 days of the postoperative period. Results: The animals from the group PP presented visceral adhesions on the mesh surface, which was not observed in the ones from group PH. At the histopathological examination foreign body response was observed in both groups, whilst there was a greater intensity of inflammatory response in group PH on both moments. Conclusion: The poli (2-hydroxyethyl methacrylate) polyHEMA hydrogel associated to polypropylene mesh reduces visceral adhesion formation in rats, although it may be associated to greater inflammatory reaction. Key words: Peritoneum. Tissue Adhesions. Hydrogel. Polymers. Surgical Mesh. Polypropylenes. Biocompatible Materials. Rats. RESUMOObjetivo: Verificar se compósito poli 2-hidroxietil dimetacrilato (PoliHEMA) / tela de polipropileno implantado na parede abdominal de ratas seria adequado para prevenção de aderências peritoneais e avaliar a resposta tecidual desencadeada por este biomaterial. Métodos: Foram implantadas telas de polipropileno -Grupo PP (n=20) e telas de polipropileno revestidas por uma camada de poli 2 (hidroxietil dimetacrilato)-PolyHEMA -Grupo PH (n=20) na parede abdominal de ratas da linhagem Wistar. Dez animais de cada grupo foram submetidos à eutanásia aos 15 e 30 dias de pós-operatório. Resultados: Os animais do grupo PP apresentaram aderências viscerais na superfície da tela, o que não foi observado nos do grupo PH. Observou-se no exame histopatológico resposta tipo corpo estranho nos dois grupos sendo que no grupo PH houve maior intensidade de resposta inflamatória nos dois momentos. Conclusão: O hidrogel de poliHEMA quando associado à tela de polipropileno reduz a formação de aderências viscerais em ratos, embora possa estar associado à reação inflamatória mais intensa.

Purpose: To evaluate whether the lining facing the visceral side of polypropylene mesh made with 2-hydroxyethyl methacrylate (p(HEMA)) hydrogel could avoid peritoneal adhesion in female dogs. Methods: Eight animals (group PP) had a polypropylene mesh implanted to correct a defect in the rectal abdominal muscle, whereas in the other group (group PH) the polypropylene mesh was coated with p(HEMA) composite on the surface facing the peritoneal area. Results: Adhesions were observed on the mesh in 62.5% of the PP group. In the PH group adhesions were present only on the suture lines. Conclusion: p(HEMA) hydrogel was well tolerated and effective in avoiding visceral and omental adhesions on the surface of the polypropylene mesh.

RESUMOA hérnia perineal acomete mais freqüentemente cães machos de meia idade e idosos, não castrados, podendo ser uni ou bilateral. A apresentação desta doença na fêmea pode ser sugestiva de alguma alteração estrutural no colágeno que leva às alterações da matriz extracelular. Sendo assim, o objetivo deste trabalho foi descrever um caso de hérnia perineal associada à colagenopatia em uma cadela. Foi examinada uma cadela Pinscher de 3 anos de idade, portadora de hérnia perineal bilateral. Foi observado hiperextensibilidade da pele. Este animal foi submetido á reparação da hérnia onde havia laceração da pele durante a dermorrafia. Foi realizada biópsia e coloração com HE e as alterações observadas foram diminuição do número de fibras colágenas na derme, que apresentavam aparente desordem na sua estrutura, notando-se raros feixes de fibras com acentuada fragmentação. Foi possível concluir que os achados clínicos e alterações histopatológicas são compatíveis com astenia cutânea na cadela. ABSTRACTMajority of cases the perineal hernia occur in intact male dogs that are middle-aged, sexually intact, and it may be unilateral or bilateral. That presentation of this illness in the bicth can be suggestive of some structural alteration in the collagen that leads to the alterations of the extracellular matrix. The purpose of this paper was to describe a case of perineal hernia associated to the collagenopathy in a bicth. An 3-years-old bitch was evaluated with perineal hernia bilateral. Hyperextensibility of the skin was observed. The perineal hernia was surgically repaired and it was observed laceration of the skin during the suture. It was carried through biopsy and HE staining. Histopathological examinations revealed alterations at the distribution and conformation of collagen fibers. There was rarefaction of connective tissue. Collagen fibers were shorten and frequently fragmented. It was possible to conclude that the clinical findings and histopathologics alterations are compatible with cutaneous asthenia in this dog.