We present 50 cm long microchannels in a monolithic device for high resolution, long read‐length DNA sequencing. These devices were fabricated and bonded in borofloat glass using unconventional photolithography techniques with 48—188 independent, straight microchannels. The microchannel DNA separation was tested with POP‐6™ polymer and a DNA sequencing ladder separated at room temperature and 200 V/cm. Single‐base resolution greater than 600 bases was achieved and the sequence base called to 640 bases with 98% accuracy. Under the same experimental conditions, the performance of the microchip was identical to a fused‐silica capillary with similar cross‐sectional area.

We present 50 cm long microchannels in a monolithic device for high resolution, long read-length DNA sequencing. These devices were fabricated and bonded in borofloat glass using unconventional photolithography techniques with 48-188 independent, straight microchannels. The microchannel DNA separation was tested with POP-6 polymer and a DNA sequencing ladder separated at room temperature and 200 V/cm. Single-base resolution greater than 600 bases was achieved and the sequence base called to 640 bases with 98% accuracy. Under the same experimental conditions, the performance of the microchip was identical to a fused-silica capillary with similar cross-sectional area.