Highlights Aging affects the metabolic profile of fibroblasts derived from ALS cases Increased NADH metabolism with age is observed in the presence of a specific set of catabolic energy substrates in healthy individuals Reduced NADH metabolism with age is observed in the presence of glycogen in the ALS cohort Disease progression rates in ALS cases correlate with fibroblast NADH production in the presence of a number of energy substrates including inosine