Aims: To understand the mechanisms by which cattle circulate and accumulate vitamin E, we cloned the cDNA for bovine α-tocopherol transfer protein (α-TTP) and examined its expression in different tissues. Methodology: A full length α-TTP cDNA was amplified from bovine liver by RT-PCR. Poly (A) + RNA obtained from bovine tissues was subjected to RT-PCR analysis to examine α-TTP mRNA expression. Western blot analysis was performed using polyclonal antibody raised against an oligopeptide derived from bovine α-TTP to examine α-TTP protein expression in various bovine tissues. The localization of α-TTP in bovine lung tissue was examined by immunostaining with antibovine α-TTP polyclonal antibody.