When ectotherms are exposed to low temperatures, they enter a cold-induced coma (chill coma) that prevents resource acquisition, mating, oviposition, and escape from predation. There is substantial variation in time taken to recover from chill coma both within and among species, and this variation is correlated with habitat temperatures such that insects from cold environments recover more quickly. This suggests an adaptive response, but the mechanisms underlying variation in recovery times are unknown, making it difficult to decisively test adaptive hypotheses. We use replicated lines of Drosophila melanogaster selected in the laboratory for fast (hardy) or slow (susceptible) chill-coma recovery times to investigate modifications to metabolic profiles associated with cold adaptation. We measured metabolite concentrations of flies before, during, and after cold exposure using NMR spectroscopy to test the hypotheses that hardy flies maintain metabolic homeostasis better during cold exposure and recovery, and that their metabolic networks are more robust to cold-induced perturbations. The metabolites of cold-hardy flies were less cold responsive and their metabolic networks during cold exposure were more robust, supporting our hypotheses. Metabolites involved in membrane lipid synthesis, tryptophan metabolism, oxidative stress, energy balance, and proline metabolism were altered by selection on cold tolerance. We discuss the potential significance of these alterations.

A linear regression model with imprecise response and p real explanatory variables is analyzed. The imprecision of the response variable is functionally described by means of certain kinds of fuzzy sets, the LR fuzzy sets. The LR fuzzy random variables are introduced to model usual random experiments when the characteristic observed on each result can be described with fuzzy numbers of a particular class, determined by 3 random values: the center, the left spread and the right spread. In fact, these constitute a natural generalization of the interval data. To deal with the estimation problem the space of the LR fuzzy numbers is proved to be isometric to a closed and convex cone of R3 with respect to a generalization of the most used metric for LR fuzzy numbers. The expression of the estimators in terms of moments is established, their limit distribution and asymptotic properties are analyzed and applied to the determination of confidence regions and hypothesis testing procedures. The results are illustrated by means of some case-studies. © 2010 Elsevier Inc. All rights reserved

Fuzzy clustering methods discover fuzzy partitions where observations can be softly assigned to more than one cluster. The package fclust is a toolbox for fuzzy clustering in the R programming language. It not only implements the widely used fuzzy k-means (FkM) algorithm, but also many FkM variants. Fuzzy cluster similarity measures, cluster validity indices and cluster visualization tools are also offered. In the current version, all the functions are rewritten in the C++ language allowing their application in large-size problems. Moreover, new fuzzy relational clustering algorithms for partitioning qualitative/mixed data are provided together with an improved version of the so-called Gustafson-Kessel algorithm to avoid singularity in the cluster covariance matrices. Finally, it is now possible to automatically select the number of clusters by means of the available fuzzy cluster validity indices.

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