A polyclonal antiserum (AS) was developed and characterized for the detection of immature stages of the black-scale, Saissetia oleae, in whole body homogenized field-collected coccinellid species, using an indirect enzyme-linked immunosorbent assay (ELISA). The indirect ELISA showed to be sensitive to the S. oleae AS, detecting a protein content between 0.118 and 0.0374 lg mL -1 . The specificity of the ELISA was tested by assaying a range of sympatric predators and alternative preys with the S. oleae AS. Coccinellid larvae obtained the highest cross-reaction and a positive-negative threshold was established at 0.674 lg mL -1 protein. A total of 1,322 coccinellids were field-collected in three olive groves located in Trás-osMontes (northeast of Portugal) by the beating technique and were analyzed to detect S. oleae proteins in their guts. Fieldcollected coccinellids which attained a S. oleae protein concentration equivalent higher than the threshold were considered as a positive reaction. In the overall collected coccinellids, 21.2% reacted positively with the S. oleae AS. Chilocorus bipustulatus and coccinellid larvae obtained the highest percentages of positives with 43.4 and 40.8%, respectively. The greatest frequency of positive responses occurred at the beginning of July, mid-August, and midOctober coinciding with the occurrence of the first, second and third instar nymphs of S. oleae, respectively. Thus, in this study, the role of coccinellids as natural control agents of S. oleae was highlighted by the number of individuals and species that tested positive for S. oleae AS.

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Metodologia de avaliação do risco associado ao galgamento de estruturas marítimas. Aplicação ao porto e à baía da Praia da Vitória, Açores, Portugal * Risk assessment methodology for the overtopping of maritime structures. application to the port and bay of Praia da Vitória, Azores, Portugal Palavras-chave: Avaliação de risco, GUIOMAR, Propagação de ondas, Modelos neuronais. ABSTRACTThe present work describes the methodology developed to assess the overtopping risk of port and coastal structures that was implemented on GUIOMAR, an integrated decision support system for port and coastal management, developed by the National Laboratory for Civil Engineering (LNEC), Portugal, whose focus is to prevent and support the management of emergency situations and the long-term planning of interventions in the study area.The incident wave climate and the corresponding mean overtopping discharges per unit length of the crest of the studied structure are the basis of the above referred methodology.The sea state determination is performed by coupling numerical wave propagation models using input wave data from wave-buoys or regional forecast wave models. The overtopping calculation is carried out with the NN_OVERTOPPING2 tool, which is based on neural network modelling. This tool was developed as part of the European project CLASH to predict Froude-scaled mean wave overtopping discharges, q, and the associated confidence intervals for a wide range of coastal structure types (such as dikes, rubble mound breakwaters, and caisson structures). In addition, prototype mean overtopping estimations, allowing for scale and model effects, are provided.The presented overtopping risk assessment methodology is based on four major steps: (i) the determination of wave overtopping over the studied structure considering the incident sea waves in the period between 2009 and 2010; (ii) the determination of the probability of overtopping exceeding pre-determined thresholds and corresponding probability levels; (iii) the establishment of the levels of consequences associated to the occurrence of overtopping that exceeds those thresholds; (iv) the combination of the above steps to assess overtopping risk levels.Once obtained the risk level, it is possible to plot it on a map, as well as the acceptability level of that risk in each studied zone or structure. This information is crucial for the management of long-term interventions on port and coastal areas.The methodology was tested for three different structure cross-sections of the port and bay of Praia da Vitória, at Terceira Island, Azores: the crosssection of the south breakwater that directly protects the dock 12 of the port, the cross-section of the seawall directly in front of the entrance of the bay of Praia da Vitória and the cross-section of the root of an existing groyne in the same area. In all studied sections, the risk of overtopping caused by the incident sea waves was characterized for the two-year period between 2009 and 2010.The presented methodology proved to be simple and effecti...

Analisar as evidências científicas publicadas sobre as estratégias e desafios em manter a saúde mental dos profissionais de enfermagem no contexto da pandemia Covid-19. O presente estudo trata de uma revisão bibliográfica do método revisão integrativa da literatura, realizado nos meses entre janeiro a maio de 2021. A busca efetuou-se, através da Plataforma da Biblioteca Virtual em Saúde - BVS, utilizando as bases de dados (LILACS), (BDENF), por meio da   (SCIELO). Aderindo-se através dos descritores/palavras chaves: “Enfermagem”, “Saúde Mental”, “Covid-19”, combinados com o operador booleano “AND”. O profissional fica exposto e pode levar à ocorrência de desgastes psicológicos, estresse elevado, ansiedade, depressão. Essas comorbidades, quando se fazem presentes, podem impactar negativamente na satisfação com o trabalho, resultando em prejuízos na assistência, qualidade do cuidado e segurança do paciente. É comum atualmente identificar sintomas de ansiedade e depressão e o grande impacto que essas manifestações causam sobre o bem-estar e as atividades diárias dos trabalhadores da saúde. Na enfermagem, percebe-se um grande índice dessas manifestações psíquicas entre os profissionais. Destaca-se que todos esses fatores estressores relacionados à atuação da enfermagem tendem a se exacerbarem diante de um cenário de calamidade como o qual têm se instalado nos últimos tempos, pois o mundo atualmente está passando por um período de turbulência decorrente da pandemia ocasionada pelo novo coronavírus. Diante desse contexto, faz – se necessário que sejam implementadas intervenções de suporte psicológico para esse grupo, tendo em vista que os profissionais de saúde correm um risco significativamente elevado e apresentam psicopatologias como: ansiedade, depressão, estresse, insônia, medo, TEPT, síndrome de Burnout.