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Background: Exercise during pregnancy is very beneficial for maternal health and fetus also. Proper knowledge in pregnant mothers is very important to promote practicing of it. Objective: To assess the knowledge and attitude of pregnant women regarding antenatal exercises. Methods: This is a cross-sectional study done in Isra institute of rehabilitation and sciences, Karachi & JPMC, Karachi. Total of 266 pregnant women during their 2nd and 3rd trimester and willing to participate in study were included from different gynecological departments of Hyderabad. Complete history was assessed regarding patient’s age, gestational age, educational status and any previous known pathology. Data were collected through face to face interviews and a validated and modified questionnaire with informed consent. For recording all the data, a designed questioner proforma was used. SPSS version 20 was utilized for data analysis, which was then summarized by descriptive statistics using frequency tables, charts and percentages. Results: Total 266 pregnant women were interviewed; the mean age was 27.86±4.79 years, with a minimum and maximum age of 18 and 44 years respectively. Majority (54.97%) of the women did not have knowledge regarding antenatal exercise. However, 79.0% of women showed positive attitude towards antenatal care. Conclusions: Majority of pregnant women had inadequate knowledge regarding antenatal exercises, while most of whom with a positive attitude.

RNA interference is a useful and efficient tool that had been used to incorporate tolerance against different stresses. Five unigene sequences were selected from exotic grain aphid that were reported to be an ideal RNAi targets. Aphids (Macrosiphum rosae, Rhopalosiphum maidis, Sitobion avenae, Toxoptera aurantii) were reared on Rosa indica, Zea mays, Triticum aestivum, Chrysanthemum hibiscus, Solanum melongena, Abelmoschus esculentus. PCR of unigene 28469 and 21789 was positive for all aphids and there is no ortholog found for them so unique and effective to be used in RNAi technology. Cytochrome c oxidase was found to be positive for M. rosae, S. avenae, T. aurantii and negative for R. maidis. Zinc finger protein was found to be positive for R. maidis, S. avenae and negative for M. rosae, and T. aurantii. Cuticular proteins was found to be positive for S. avenae and M. rosae and negative for R. maidis and T. aurantii. Genes identified in the aphids are defense and as important structural genes and their suppression with RNAi technology will be important target to have insect resistant crops. From these gene sequences cytochrome c oxidase is reported as bar coding gene and can be used in future for interspecific genetic variation in these aphids. Results revealed the high expression of these sequences in local aphid species and can be used as RNAi target for them. That can be used in future or applications as pest management, monitoring and plant quarantine.

Objective: The main goal of this study was to investigate the groups receiving fentanyl-propofol (fentP) against ketamine-propofol (ketP) in ERCP in terms of sedation, rescue sedation requirement, and recovery scores. Additionally, evaluated were the procedure's hemodynamic changes, postoperative pain score, complications, and endoscopist satisfaction. Methodology: A double-blinded randomized clinical trial was undertaken at the Dr. Ruth K.M. Pfau Civil Hospital Karachi's endoscopic room (DUHS) for six months. By using OPEN EPI sample size calculator, sample size was calculated. A total of 124 patients for elective ERCP were randomized into two groups by SNOSE protocol. Groups A and B, fentanyl-propofol (fentP) and ketamine-propofol (ketP), respectively, each contain 62 patients. All patients were given a loading dose of propofol 0.5 mg/kg, followed by a 75 ug/kg/minute infusion. The group fentP received fentanyl 1ml/kg (1 ug/kg) and the group ketP received ketamine 1ml/kg (0.5mg/kg). Ramsay sedation scores, the necessity for rescue sedation, and the Aldrete score post-operatively were noted. Hemodynamics during surgery and complications were also noted. Results: Sedation began noticeably earlier than usual in the group B at 0, 2 and 4 minutes (p-value <0.05), whereas sedation scores were higher in the group A at 8,10, and 15 minutes (p-value <0.05). Early sedation in the group B led to less consumption of rescue sedation doses (p-value <0.01). However, recovery scores were comparable in each groups (p-value >0.05). Conclusion: We were able to conclude that during ERCP, ketP had a significantly faster sedative onset than fentP, with less complication and a quicker recovery. Keywords: Propofol, KetP, FentP, ketamine, fentanyl, Sedation, Analgesia, ERCP, Monitored Anesthesia care.

Blood donation acts as a life saving measure and steps are required to remove misunderstanding about current issues. Blood donation is required every second in life globally. Purpose: To determine and evaluate an attitude and knowledge of people regarding blood donation. Study Design: Descriptive cross sectional study. Methodology: Present study was conducted among the employees of CMH Kharian Medical College over a time period of 3 months. Data was collected by self structured questionnaire through convenient sampling. Data analysis was done by SPSS 20. Results: Out of 180 participants, 87% had donated blood in the past, 81.66% were willing to accept blood donation. Most of the participants were unafraid of donating blood (83.34%). Only 35% thought that they will catch infection while 71.67% believed that they will become obese, unconscious and anemic. Conclusion: We concluded that there was a positive attitude among the respondents towards blood donation. However, survey showed that blood donation (voluntarily & regularly) was low in actual practice according to WHO standards. Most likely reasons included misconception related to it. Hence, government should plan a strategy to educate common people regarding this health issue. Key word: Blood Donation, Attitude, Health Issue and Employees.