The Estação Biológica Santa Lúcia (EBSL) is one of the oldest reserves in Brazil, used as a research site since 1939 by the naturalist Augusto Ruschi. It is an Atlantic Forest fragment, and its fauna and flora have been studied throughout the years. However, its chiropteran fauna remains virtually unknown. Here, we aim to provide a bat species list for EBSL. We installed mist-nets over 19 nights from mid-2009 to mid-2010, with a sampling effort of 20 875.5 m2.h. We captured 204 specimens, from two families and 22 species. Ecological analysis reveals a medium to high diversity, heavily dominated by frugivorous species. The collector’s curve and the estimative of species richness suggests that the chiropteran fauna at EBSL remains partially unknown, and we encourage further inventories.