Background: Situational hypertension and differences between devices complicate interpretations of systolic blood pressure (SBP) measurements in dogs. Hypothesis/Objectives: To evaluate if time point of in-clinic SBP measurement, type of oscillometric device, and operator affect SBP measurements in conscious dogs. Animals: Sixty-seven privately owned dogs with or without chronic kidney disease, divided into 2 study samples (A and B). Methods: Cross-sectional diagnostic study. In part A, SBP measurements in dogs were performed using 2 different devices (HDO and petMap) after acclimatization at 3 standardized time points during a clinical visit. In part B, SBP measurements (HDO) were performed in dogs by a trained final year veterinary student and by the owner alone, at the same occasion. Results: For all dogs, there was no difference in mean SBP (mSBP) among the 3 time points for HDO (P = .12) or petMAP (P = .67). However, intraindividual mSBP differences of up to 60 mm Hg between time points were documented. Mean SBP obtained with petMAP was on average 14 (95% CI: 8-20) mm Hg higher than mSBP obtained with HDO, and this difference increased with increasing SBP. Mean SBP measurements obtained by the trained student were 7 (95% CI: 2-11) mm Hg higher than mSBP measurements obtained by the owner. Conclusions and Clinical Importance: According to the results of this study, time point of in-clinic SBP measurement in dogs is of minor importance, and instructing owners to perform measurements might reduce suspected situational hypertension. Abbreviations: ACVIM, American College of Veterinary Internal Medicine; BP, blood pressure; CKD, chronic kidney disease; mSBP, mean systolic blood pressure calculated from multiple measurements with a variation of ≤20%; mSBPowner, mean systolic blood pressure calculated from measurements performed by the owner; mSBPowner_adjusted, mean systolic blood pressure calculated from measurements performed by the owner after exclusion of unreliable measurements as indicated by the HDO software; mSBPvet, mean systolic blood pressure calculated from measurements performed by the trained veterinary student; mSBPvet_adjusted, mean systolic blood pressure calculated from measurements performed by the veterinary student after exclusion of unreliable measurements as indicated by the HDO software; SBP, systolic blood pressure; TP, standardized time point during a clinical visit.