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The quantitative determination of aliphatic alcohols In liquid chromatography has been hindered by the lack of a sensitive detector. Aliphatic alcohols have no Inherent chromophore or fluorophore and are considered to be electrolnactlve under constant applied potential. The determination of underlvatized aliphatic alcohols by reversed-phase polymer-based chromatography with pulsed amperometric detection (PAD) Is direct, sensitive, and simple. PAD was applied to the determination of simple alcohols at Pt and Au working electrodes under acidic and basic conditions, respectively. The combination of mixed-mode chromatography with a chromophoric (UV-vls) detector and a nonchromophoric detector (PAD) is a powerful analytical technique.

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