Bacterial resistance to exis ng an bio cs has driven a search for new an bio cs from marine ac nobacteria. Bioac vity and gene c screening of ac nobacteria associated with red algae Gelidiella acerosa were conducted to discover new an bacterial compounds against Vibrio alginoly cus. A total of 14 ac nobacteria isolates were obtained from G. acerosa. The isolates were subjected to gene c screening for nrps (non-ribosomal pep de synthetase) and FADH 2 -dependent halogenase genes. The isolates' ability to produce secondary metabolites was examined by fermenta on in various media in a six-well mini plate. The bioac vity of the secondary metabolites was screened using a micro ter assay and the agar overlay method. The results showed that all 14 isolates had the nrps gene, whereas none had the halogenase gene. Meanwhile, eight of the ac nobacteria isolates showed an bacterial ac vity against V. alginoly cus.

Seaweeds are macroscopic, multicellular, eukaryotic and photosynthetic organisms, and are a source of chemical diversity with powerful biological activities for diversified industrial applications including cosmeceuticals. Red seaweeds (Rhodophyta) are good sources of Mycosporine-like amino acids (MAA) for photoprotectant and antiphotoaging compounds. In addition, Rhodophyta are also good sources for hydrogel compounds that are used widely in the food, pharmaceutical and cosmeceutical industries as gelling agents, moisturizers or for their antiphotoaging effects. Our survey and ongoing studies revealed that the biodiversity of Indonesian Rhodophyta is rich and is a treasure trove for cosmeceutical agents including MAA and hydrogels. This study delivers valuable information for identifying potential red seaweeds in screening and searching for cosmeceutical agents.

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Vibriosis causes a problem in aquaculture and human health. The antibiotic resistance and the limited availability of antimicrobial agents are the driving force for searching for new antivibrio compounds from natural products. Herein, we evaluated ten marine Actinobacteria isolated from red seaweed Gelidiella acerosa for antivibriosis, cytotoxicity and secondary metabolites genes in the way to find a new antivibrio agent. Five Actinobacteria isolates exhibited activity against Vibrio alginolyticus and Vibrio parahaemolyticus with MIC ranging from 0.625 to 5 µg/µl. All isolates displayed no cytotoxicity against Vero cell line except for isolate DR‐2R‐115–8. All isolates harbour nrps gene, which might indicate that these isolates have great potential in the production of bioactive peptides while none of the isolates showed the presence of pks I, II and hmgcr genes. Identification of ten isolates of marine Actinobacteria associated with red algae was classified into four genera of Brevibacterium, Nocardiopsis, Allokutzneria and Brachybacterium. Our study revealed that the marine Actinobacteria associated with red algae Gelidiella acerosa were promising sources for controlling vibriosis.

BACKGROUND: After hemodialysis, complications may occur and one of them that problematic is hemodialysis-related nausea. Information is still unclear about aromatherapy concerning nausea-related to hemodialysis. This study aimed to describe the effect of aromatherapy on nausea after hemodialysis procedures. CASE PRESENTATION: A 51-year-old female, undergoing routine hemodialysis, experienced severe hemodialysis-related nausea. Her nausea occurred a day after dialysis and existed for 3–4 days and this repeated as a cycle that affected her appetite, food intake, and general condition. The daily measurements of nausea used the visual analog scale and the nausea severity scale before and after the intervention. In addition, a manual book with a packet containing nausea education, protocol of intervention, and monitoring sheets, was provided. For two weeks, the application of two drops of lavender aromatherapy was given on a humid cotton swab and inhaled for 5 min. In addition, education explained oral hygiene using warm water when waking up in the morning and before eating food. There was a decrease in the intensity of nausea from severe to none based on daily measurements and from severe nausea (score: 21) to moderate nausea (score: 10) after the 2-weeks intervention. Thus, the patient could enjoy eating without any uncomfortable feeling of nausea. CONCLUSION: Lavender aromatherapy was demonstrated as a complementary therapy in overcoming nausea after dialysis among CKD patients. This report could provide a novel therapeutic modality for hemodialysis patients suffering hemodialysis-related nausea.