Objective: Cytochrome b5 reductase (CYB5R) and cytochrome P450 reductase (CYPOR) play an important role in cell metabolism; however, their role in thyroid hormonogenesis and carcinogenesis has not been studied. The activity of CYB5R correlates with metastasizing in breast cancer, but there are no similar studies for CYB5R and CYPOR for thyroid cancer. The aim was to assess the activity of CYB5R and CYPOR in thyroid tissues in benign and malignant thyroid neoplasms.Methods: 36 patients with thyroid neoplasms participated in the study. The control euthyroid goiter group included 10 patients; the thyrotoxic nodular goiter group included 14 patients; the papillary thyroid cancer T1-2N0-1M0 (PTC) group included 12 patients. The activity of CYB5R and CYPOR was assessed with lucigenin-enhanced chemiluminescence stimulated by NADH and NADPH, respectively. Results: The activity of CYB5R and CYPOR increased several times in thyrotoxicosis andapproximately by an order of magnitude in some cases of PTC, but the activity change of CYPOR was more pronounced compared to CYB5R. For the PTC group, the subgroups with low and high activity of microsomal reductases were detected. Microsomal reductases in follicular adenoma was 2-4-fold less active compared to nontoxic goiter and the low-activity PTC group.Conclusions: Activity of microsomal reductases varies in thyroid pathology and can serve as a diagnostic and prognostic parameter in papillary thyroid cancer.

Objective: Cytochrome b5 reductase (CYB5R) and cytochrome P450 reductase (CYPOR) play an important role in cell metabolism; however, their role in thyroid hormonogenesis and carcinogenesis has not been studied. The activity of CYB5R correlates with metastasizing in breast cancer, but there are no similar studies for CYB5R and CYPOR for thyroid cancer. The aim was to assess the activity of CYB5R and CYPOR in thyroid tissues in benign and malignant thyroid neoplasms. Methods: 36 patients with thyroid neoplasms participated in the study. The control euthyroid goiter group included 10 patients; the thyrotoxic nodular goiter group included 14 patients; the papillary thyroid cancer T1-2N0-1M0 (PTC) group included 12 patients. The activity of CYB5R and CYPOR was assessed with lucigenin-enhanced chemiluminescence stimulated by NADH and NADPH, respectively. Results: The activity of CYB5R and CYPOR increased several times in thyrotoxicosis and approximately by an order of magnitude in some cases of PTC, but the activity change of CYPOR was more pronounced compared to CYB5R. For the PTC group, the subgroups with low and high activity of microsomal reductases were detected. Microsomal reductases in follicular adenoma was 2-4-fold less active compared to nontoxic goiter and the low-activity PTC group. Conclusions: Activity of microsomal reductases varies in thyroid pathology and can serve as a diagnostic and prognostic parameter in papillary thyroid cancer.

Background: The analysis carried out and the forecasted development prospects of the Vsevolozhsk municipal district show the high socio-economic importance of this territory for St. Petersburg and the Leningrad region. This territory is intensively developing, new residential zones are being formed on it, enterprises are opening, new jobs are being created, and recreational zones are being organized. Active housing construction in the municipalities "City of Vsevolozhsk" and "Zanevskoye rural settlement" is accompanied by a significant increase in the population. According to the forecast, in 2041 the population of the municipal formation "City of Vsevolozhsk" will exceed 100 thousand people, of the "Zanevsky rural settlement" - 180 thousand people. The population of the municipalities of St. Petersburg included in the gravitational zone of the maglev transport line in the direction "Vsevolozhsk - St. Petersburg", in 2041 will increase by 1.4 times compared to 2015, amounting to 520 thousand people. The listed tendencies indicate that in the future the intensity of traffic flows will grow and the demand for the use of maglev passenger transport will increase. Aim: Putting into operation lines of magneto-transport vehicles in places of concentration of growing passenger flows, which will help to reduce travel time, meet freight requirements, improve the quality and increase travel safety when driving along dedicated lanes. Method: We have described the existing transport service system in Vsevolozhsk. The survey of passenger traffic on bus routes operating between Vsevolozhsk and St. Petersburg was carried out by two methods during the periods of morning and evening peaks, as well as during the inter-peak period: visual method and tabular method. The article gives a characteristic of the uneven distribution of passenger traffic at the Ladozhskaya metro station and the socio-economic characteristics of the pedestrian accessibility zone of stopping points, identifies the maximum passenger traffic and the need for rolling stock. Results: As the basis for the development of the transport system of urban agglomerations, it is proposed to use magnetolithic transport. For its operation, a special high-speed infrastructure and a new rolling stock are needed. In other words, there is a need for the development and economic evaluation of projects for the construction and operation of magnetolithic transport lines in the formation and implementation of transport strategies of modern urban agglomerations.

The aim of the study was to investigate the activity of NADH-dependent cytochrome b5 reductase (CYB5R) and NADPH-dependent cytochrome P450 reductase (CYPOR) in ovarian tissues after cryopreservation by lucigenin-enhanced chemiluminescence with NADH and NADPH stimulation, respectively. The results indicate that both mitochondrial and microsomal reductase activities are preserved in cryopreserved ovarian tissues. After cryopreservation, the level of production of superoxide anion radical by mitochondria drops by 3–10 times, while the presence or absence of chemotherapy has no effect, and this parameter is also not affected by the severity of the disease. Compared to the control group (patients with benign tumors), the activity of CYB5R and CYPOR of ovarian tissue in a malignant cancer process decreases 1.5–10 times depending on the disease severity, and the presence of chemotherapy dramatically affects these parameters — the activity of microsomal reductases decreases by 50–100 times in chemotherapy compared to the control group. Thus, microsomal reductase activity is a more informative parameter for assessing the functionality of cryopreserved ovarian tissue than superoxide-producing capacity of mitochondria, because, firstly, it depends on the stage of disease and prior chemotherapy, and secondly, the analytical signal of NADH/NADPH stimulated chemiluminescence is characterized by approximately 30 times higher intensity than mitochondrial chemiluminescence, which leads to higher analytical sensitivity of the technique.