2 Klinika za neurologiju i psihijatriju za decu i omladinu, Beograd 3 Institut za zdravstvenu zaštitu majke i deteta Srbije "Dr Vukan Čupić", Beograd 4 Institut za statistiku i informatiku, Medicinski fakultet u Beogradu Apstrakt Spinalna mišićna atrofija (SMA) je autozomno recesivna neuromišćna bolest koju odlikuje progresivna denervacija skeletnih mišića usled propadanja alfa-motoneurona i sledstvena pojava hipotonije, mišićne slabosti, atrofije. Cilj rada je da se pokaže potencijalni uticaj broja kopija SMN2 na razvoj jednog od tri tipa SMA. Ispitivanjem je obuhvaćeno 74 pacijenta oba pola sa kliničkom dijagnozom SMA i to 44 (59.4%) ženskog pola i 30 (40.5%) muškog pola. Pacijenti su klasifikovani u određenu kategoriju SMA (tip 1, 2 ili 3). Glavni kriterijum za klasifikaciju bio je mogućnost samostalnog sedenja/hodanja. Dijagnozu SMA tip 1 imalo je 8 (11.3%) pacijenata, SMA tip 2 27 (38%), dok je SMA tip 3 imalo 36 (50.7%). Homozigotna delecije gena SMN1 bila je zastupljena kod 72/74 (97.2%) pacijenta, dok je kod preostalih detektovana jedna kopija ezgona 7 i 8 SMN1 gena (heterozigotni status), u kombinaciji sa tačkastom mutacijom na drugom alelu. Broj kopija SMN2 gena je bio statistički značajno niži kod SMA tip 1 pacijenta nego kod SMA tip 2 i tip 3 pacijenata (p<0.001). Broj kopija gena SMN2 je u korelaciji sa težinom kliničke slike SMA, što se može koristiti kao jedan od prognostičkih parametara kod ovog obolenja. Ključne reči: SMA, SMN1, broj kopija SMN2 gena, homozigotna delecija.

Introduction: Clinical and morphological prognostic factors for PCa are Gleason score, TNM classification and lymph node invasion. VEGF is an inducer of angiogenesis, which plays an important role in tumor growth and metastasis. It was shown that PCa cells express VEGF. Aim: The aim of this paper is to analyze the immunohistochemical expression of VEGF in prostate adenocarcinoma and correlate it with the clinical and morphological prognostic factors in the sample of patients treated at the Clinical Hospital Center "Dr Dragiša Mišović" in Belgrade. Material and methods: The operative material from 20 cases of prostate adenocarcinoma was used. TNM classification from 2002 was used to determine the stage of the tumor, and Gleason score from 2009 was applied to determine the grade. Immunohistochemical staining of prostate adenocarcinoma was semiquantitatively evaluated. Results: Expression of VEGF was present in all analyzed adenocarcinoma of the prostate. In fifteen cases (75%) of the analyzed twenty cases, immunostaning was diffuse, while in five cases it was moderate (25%). A statistically significant correlation was noted between diffuse expression of VEGF and higher Gleason score (p < 0.01). Frequency of diffuse expression of VEGF was higher in cases with the presence of lymphatic node metastasis and TNM stage III and IV, but the results were not statistically significant. Conclusion: Diffuse expression of vascular endothelial growth factor (VEGF) is more often observed in prostate cancer (PCa) with higher Gleason score, higher TNM stage and lymph node invasion, in the sample of patients treated at the Clinical Hospital Center "Dr Dragiša Mišović" in Belgrade, all consistent with previous studies.