ABSTRACT. Knowledge of specific hematological parameters of laboratory animals used in experiments is highly relevant to evaluate functional alterations of their vital organs. Hematological reference rates for rats, mice, rabbits and hamsters, bred in conventional healthy conditions and derived from the Vivarium of the FMUSP's Vivarium Center are established. One hundred and twenty mice of the strains BALB/c, C57BL / 6 and Swiss, 80 Wistar rats, 80 Golden hamsters and 80 New Zealand rabbits, males and females, apparently normal and healthy, over 10-weeks-old, were employed in current experiment. The analysis was determined by flow cytometry process (CELM CC510) involving the separation of erythrocytes and leukocytes, and cell identification by the suspension method and quantification. Hematological derivatives such as hematometric indexes (application of equations) and morphological quantification of leukocytes and erythrocytes were determined by scanning/differential (Panótico-commercial), whereas hemoglobin dosage was determined by spectrophotometry (CELM E210D). Results are given as means and standard deviants of hematological profiles in the blood of the animals grouped by species, strain and sex. Data are an important asset for researchers in their analysis of experimental results. CEAU Certificate n.° 251/11 HCFMUSP. Keywords: hematological rates, blood, sanitary conditions, animals.Intervalos de referência para alguns parâmetros hematológicos de animais criados e mantidos pelo Biotério da Faculdade de Medicina da Universidade de São Paulo RESUMO. Conhecer os parâmetros hematológicos individuais de animais de laboratório utilizados na experimentação é importante para avaliar as alterações funcionais dos seus órgãos vitais. Este trabalho estabelece valores de referência hematológicos de roedores e lagomorfos criados em condições sanitárias convencionais, provenientes do Biotério do Centro de Bioterismo da FMUSP. Foram utilizados 120 camundongos das linhagens BALB/c, C57BL/6 e Swiss, 80 ratos Wistar, 80 hamster Golden e 80 coelhos New Zealand, machos e fêmeas, aparentemente normais e saudáveis, todos acima de 10 semanas. A análise foi determinada pelo processo de citometria de fluxo (CELM CC510) para a separação de eritrócitos e leucócitos, e a identificação celular pelo método em suspensão e quantificação. Os derivados hematológicos como índices hematimétricos (aplicação de equações), a quantificação morfológica dos leucócitos e eritrócitos foram determinados por varredura/diferencial (Panótico-comercial) e a dosagem de hemoglobina por espectrofotometria (Celm E210D). Os resultados estão apresentados pelas médias e pelos erros padrões dos perfis hematológicos encontrados no sangue dos animais, os quais estão agrupados por espécie, linhagem e sexo. Os perfis servirão como valiosa ferramenta aos pesquisadores na análise de seus resultados experimentais. Certificado CEAU n° 251/11 HCFMUSP.
4.7; P<0.003) and females (z > 6.2; P < 0.0003), when compared to control groups. As for MN cells, there were no differences between inoculated and control groups, in males (z = 2.3; P = 0.0107) and in females (z = 1.8; P = 0.0359) as well. In conclusion, these results show that inbred F344 rats are adequate biologic models for studies of acute, intra-abdominal sepsis.]]>
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